Molecular identification of Anaplasma platys in cattle by nested PCR

Mohammed  Al-Saadi; Dhama Al-Sallami; Amjed Alsultan

doi:10.18502/ijm.v15i3.12904

Mohammed Al-Saadi Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
Dhama Al-Sallami Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
Amjed Alsultan Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq

DOI: https://doi.org/10.18502/ijm.v15i3.12904

Keywords: Anaplasma platys; Cattle diseases; Tick-borne diseases; 16S rRNA

Abstract

Background and Objectives: Anaplasmosis is a zoonotic disease caused by Gram- negative bacterium from Anaplasmata- ceae family. Anaplasma causes high economic losses worldwide. 16S rRNA analysis was used to diagnose Anaplasma platys in Cattle. Phylogenetic tree and estimation of evolutionary divergence between A. platys isolates were performed.

Materials and Methods: A total of 60 blood samples were collected from a cattle farm in AL- Diwaniyah province. 16S rRNA gene was identified using nested PCR. Overall, 40% of cattle that were chosen to collect the blood were identified to be infected with A. platys.

Results: The results have shown presence of targeting partial region of 16S rRNA gene in 24 samples out of 60. Sequenc- ing results of 10 samples have revealed that the phylogenetic tree was divided in to two separate clades. Five isolates of A. platys- Iraq (accession no. OP646782, OP646783, OP646784, OP646790, and OP646791) were located in one clade with the A. platys- China (accession no. MN193068.1). While, five isolates (accession no. OP646785, OP646786, OP646787, OP646788, OP646789) were in different clade with two isolates of A. platys- Africa and A. platys- Zambia in distinct branch- es, close to the Rickettsiales.

Conclusion: The phylogenetic study of A. platys sequences indicated that the isolates were collected from a cattle farm in Al- Dewaniyah were similar and close related to A. platys- China, A. platys- Zambia and A. platys- Africa). This study suggests that cattle can be considered a reservoir of A. platys.