Inhibition of bacterial adhesion and anti-biofilm effects of Bacillus cereus and Serratia nematodiphila biosurfactants against Staphylococcus aureus and Pseudomonas aeruginosa

Alireza Keyhanian; Mojgan Mohammadimehr; Farshad Nojoomi; Hamed Naghoosi; Mehdi Shakouri Khomartash; Mohsen Chamanara

doi:10.18502/ijm.v15i3.12903

Alireza Keyhanian Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran
Mojgan Mohammadimehr Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran
Farshad Nojoomi Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran
Hamed Naghoosi Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran
Mehdi Shakouri Khomartash Medical Biotechnology Research Center, Aja University of Medical Sciences, Tehran, Iran
Mohsen Chamanara Toxicology Research Center, Aja University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijm.v15i3.12903

Keywords: Bacterial adhesion; Biofilms; Bacillus cereus; Surface-active agents; Staphylococcus aureus; Pseudomonas aeruginosa

Abstract

Background and Objectives: Biosurfactants are amphiphilic surface-active agents that mainly produced by various micro- organisms. In this study, the anti-biofilm and inhibition of bacterial adhesion activities of two bacterial biosurfactants were investigated.

Materials and Methods: After extraction and evaluation of Bacillus cereus and Serratia nematodiphila biosurfctants, inhi- bition of bacterial adhesion and anti-biofilm effects of them on Staphylococcus aureus and Pseudomonas aeruginosa were determined.

Results: On average, the synergistic effect of two bacterial biosurfactants, caused about 60% decrease in adhesion and about 80% decrease in biofilm formation of S. aureus and P. aeruginosa.

Conclusion: The results of this study showed that combination of B. cereus and S. nematodiphila biosurfactants would in- crease the potential of attachment inhibition and biofilm eradication with very low toxicity.