Bacterial profile and their antimicrobial resistance patterns among patients with community‑acquired pneumonia in southwestern Iran

Sajad Hassanzadeh; Seyed Sajjad Khoramrooz; Farzad Mazloomirad; Asghar Sharifi; Narges Roustaei; Mohammad Gholamnezhad; Esmaeel Jamshidnejad

doi:10.18502/ijm.v15i3.12894

Sajad Hassanzadeh Department of Internal Medicine, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
Seyed Sajjad Khoramrooz Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Farzad Mazloomirad Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
Asghar Sharifi Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Narges Roustaei Department of Epidemiology and Biostatistics, School of Health and Nutrition Sciences, Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Mohammad Gholamnezhad Department of Internal Medicine, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
Esmaeel Jamshidnejad Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran

DOI: https://doi.org/10.18502/ijm.v15i3.12894

Keywords: Community-acquired pneumonia; Pathogenic bacteria; Antibiotic susceptibility pattern

Abstract

Background and Objectives: Community-acquired pneumonia (CAP) is one of the most common life-threatening infec- tions, occurring in the community or within the first 48 hours of a patient's hospitalization. The present study aimed to investigate the frequency of pathogenic bacteria and their antibiotic resistance pattern in the sputum of patients with commu- nity-acquired pneumonia in Yasuj from 2018 to 2019.

Materials and Methods: In the present study, 128 patients with CAP were included. Under aseptic conditions clinical samples including sputum collected from each patient were sent to the Microbiology Laboratory. Specific culture media and biochemical tests were used to identify the bacteria. Antimicrobial resistance patterns of the isolates were examined by disc diffusion. DNA was extracted from sputum using the phenol-chloroform method. The PCR method was used for the molec- ular detection of bacteria. Data were analyzed using SPSS software version 22 and the chi-square test.

Results: The most common clinical symptoms in patients were sputum (68.8%), fever (64.1%), shortness of breath (60.2%), cough (50.8%), and chest pain (24.2%). A total of 133 bacteria were identified by culture and 117 bacteria by PCR. In the current study, the most prevalent organisms were Streptococcus pneumoniae (24.1%), Hemophilus influenzae (18%), Staph- ylococcus aureus (13.5%), and Moraxella catarrhalis (11.4%). Antibiogram test showed that most of the Gram-negative bacteria were resistant to levofloxacin (22.6%), rifampin (20.8%) and ceftriaxone (17%), and the highest resistance rate to clindamycin (43.1%), ciprofloxacin (43.1%) and amoxicillin (41.4%) were detected in the Gram-positive bacteria. Cefepime was the most effective antibiotic against Gram negative bacteria.

Conclusion: S. pneumoniae was the most prevalent bacteria identified by culture and PCR methods in patients with CAP, indicating an important role of this bacterium in the pathogenesis of CAP. According to the results, cefepime can be used to treat patients with CAP with Gram-negative bacteria. In the present study, S. pneumoniae, S. aureus, P. aeruginosa, H. influenzae, M. catarrhalis, and K. pneumoniae have been isolated from the CAP patient population with varying frequencies. This is consistent with various studies in different parts of the world.