Effect of new methionine-based ionic liquid on the CDR1 and CDR2 gene expression on sensitive and resistant strains of Candida albicans

Mona Eslami; Ali Ezabadi; Mansour Bayat; Behin Omidi; Pejman Mortazavi

doi:10.18502/ijm.v15i2.12485

Mona Eslami Department of Pathobiology, Faculty of Veterinary Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Ali Ezabadi Department of Chemistry, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Mansour Bayat Department of Pathobiology, Faculty of Veterinary Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Behin Omidi Department of Biology, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Pejman Mortazavi Department of Pathobiology, Faculty of Veterinary Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

DOI: https://doi.org/10.18502/ijm.v15i2.12485

Keywords: Ionic liquid; Candida albicans; Candidiasis; Antifungal agents; Methionine

Abstract

Background and Objectives: Previous researchers showed the antimicrobial ability of ionic liquids (ILs) on different infec- tive agents. ILs can dissolve organic components, especially DNA molecules. Among synthesized eight binary ILs mixtures, we have chosen ([Met-HCl] [PyS]) IL for determining the antifungal ability of IL against Candida albicans cells.

Materials and Methods: Well diffusion assay, chrome agar and Germ tube tests were used to detect the Candida samples. PCR, real-time-PCR, and flow cytometry tests were performed to determine the IL's rate of toxic ability.

Results: Well diffusion assay revealed the diameters of the growth inhibition zones were the largest in IL with methionine and Proline amino acids. Minimum inhibitory concentration (MIC) and the Minimum fungicidal concentration (MFC) tests showed that they inhibited the growth of the C. albicans at a range from 250 µg/ml for sensitivity and 400 µg/ml for resis- tance, MIC average of all samples were 341.62 ± 4.153 µg/ml. IL reduced the expression of CDR1 and CDR2 the genes encoded by the major protein of ABC system transporter by 2.1 (P= 0.009) and 1.2 fold (P= 0.693), revealed by PCR and real time-PCR. In the flow cytometry test, there were increasing dead cells after treating with the ([Met-HCl] [PyS]) even in the most resistant strain.

Conclusion: The novel IL was effective against the most clinical and standard C. albicans.