Detection of genes encoding enterotoxins (SEA-D) in Staphylococcus aureus strains isolated from workers’ nasal samples and creamy pastries of Shiraz confectioneries

Aliasghsr Masihinejad; Mojtaba Bonyadian; Mohammad Motamedifar

doi:10.18502/ijm.v15i2.12477

Aliasghsr Masihinejad Department of Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Mojtaba Bonyadian Department of Health and Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Mohammad Motamedifar Department of Microbiology, Shiraz University of Medical Sciences, Shiraz, Iran

DOI: https://doi.org/10.18502/ijm.v15i2.12477

Keywords: Staphylococcus aureus; Creamy pastry; Enterotoxin genes; Antibiotic resistance

Abstract

Background and Objectives: Staphylococcus aureus is responsible for the majority of food poisoning all around the world. Nasal carriers of S. aureus and foodstuffs need for handling are important sources and vehicles to transmit this pathogen to ready-to-eat foods. According to hygienic standards, confectioners should not be contaminated with S. aureus. This study aimed to detect nasal carriers and creamy pastries contaminated with enterotoxigenic S. aureus in confectioneries of Shiraz, Iran.

Materials and Methods: From the confectioneries of Shiraz city, 27 places in the north, south, center, west, and east areas were selected randomly then 100 creamy pastries samples and 117 nasal swabs were collected. Bacteriological and biochem- ical tests were performed to isolate S. aureus. The polymerase chain reaction (PCR) test was used to identify the virulence and enterotoxins genes of the S. aureus isolates. Agar disk diffusion was performed to find out the antibiotic resistance of the isolates.

Results: Results revealed that 16.24 and 33 percent of workers and creamy pastries were contaminated with S. aureus respec- tively. Also, 100%, 37%, 58%, and 6% of nasal samples harbored femA, mecA, sea, and sec genes respectively. According to the results 97%, 70%, 54.5%, and 6% of creamy pastries isolates harbored femA, mecA, sea, and sec genes respectively. No isolate carried seb and sed genes. The results also showed that 41.5% of nasals and 5.5% of creamy pastry isolates har- bored both sea and sec genes. The sea was the most common enterotoxin gene observed in nasal and creamy pastries. The results of the antimicrobial resistance test showed that 68.42% of nasal and 48.48% of creamy pastry isolates were resistant to cefocxitn (FOX) respectively. Both nasal (89%) and creamy pastry (82%) isolates presented the highest resistance to pen- icillin (P) and the most sensitivity to trimethoprim-sulphamethoxazole (SXT) (94%). Most of the isolates were sensitive to erythromycin (E), aztreonam (AZM), tetracycline (TE), trimethoprim (TMP), and ciprofloxacin (CP). Isolates of S. aureus harboring multi-enterotoxin genes were resistant to more antibiotics than others.

Conclusion: The presence of enterotoxigenic S. aureus in the workers’ nasal samples and creamy pastries of Shiraz confec- tioneries was high which is a potential public health hazard.