Inhibitory effects of postbiotic consisting sonication-killed Bifidobacterium bifidum on experimental triple negative breast neoplasm in mice: a preliminary study
Abstract
Background and Objectives: Breast cancer is the second leading cause of death and one of the most common malignancies among women in the world. Theaim of this study was to investigate the preventive effects of postbiotic consisting of soni- cated Bifidobacterium bifidum cells on triple negative breast cancer.
Materials and Methods: Thirty-six female BALB/c mice aged 5-7 weeks were randomly divided into 3 groups (n=12): Ctrl-, healthy mice; Ctrl+, mice withbreast cancer with no treatment; and Postbiotic, mice with orally gavage postbiotic before and after 4T1 cell line transplantation. Cancer progress and theeffects of postbiotic were assayed by histological, immunohistochemical and gene expression quantification.
Results: The histological results showed that administration postbiotic consisting of B. bifidum significantly decreased car- cinogenesis in terms of tumorincidence, multiplicity and volume. The tumor progress was suppressed by oral intake of B. bifidum as showed by p53 and Ki-67 expression. Furthermore, Oralintake of postbiotic resulted in extended survival of mice and inhibited sever weigh loss.
Conclusion: Pretreatment with sonication killed B. bifidum, as a postbiotic, inhibited breast cancer progress and malignancy.