Frequency of adenovirus serotype 8 in patients with Keratoconjunctivitis, in Ahvaz, Iran

Kolsoom  Shafiei; , Manoochehr  Makvandi; Ali  Teimoori; Alireza  Samarbafzadeh; Gholamreza Khataminia; Shahram  Jalilian; Niloofar  Neisi; Kimia  Makvandi; Mehrdad  Sadeghi Haj

doi:10.18502/ijm.v11i2.1074

Kolsoom Shafiei
, Manoochehr Makvandi
Ali Teimoori
Alireza Samarbafzadeh
Gholamreza Khataminia
Shahram Jalilian
Niloofar Neisi
Kimia Makvandi
Mehrdad Sadeghi Haj

DOI: https://doi.org/10.18502/ijm.v11i2.1074

Keywords: Human adenovirus; Polymerase chain reaction; Keratoconjunctivitis; Serotype

Abstract

Background and Objectives: Adenoviral keratoconjunctivitis is an extremely frequent ophthalmological disease caused by various serological subtypes of human adenovirus (HAdV) worldwide. Adenoviruses serotypes 8, 11, 19, 37 frequently cause epidemic keratoconjunctivitis (EKC). This study was conducted to evaluate the frequency of adenovirus serotypes in patients with EKC in Ahvaz , Iran.

Materials and Methods: Eighty- eight ocular swabs were collected from patients with EKC. The specimens were analyzed for detection of adenovirus by standard PCR. The PCR products were further sequenced and analyzed to determine the serotypes.

Results: The study population consisted of 49/88 (55.7%) males and 39/88 (44.3%) females. Among them 25 (51.02%) males and 22 (56.41%) females were positive for HAdV serotype 8 (p= 0.488). Overall forty-seven (53.4%) samples were positive for AdV serotype 8 while forty- one patients (46.59%) were negative for the adenovirus serotypes.

Conclusion: The results of this study revealed predominanance of HAdV 8 with high prevalence of 53.4% among patients with Keratoconjunctivitis. Forty- one patients (46.59%) were negative for adenovirus. Still, the role for other related viruses such as enteroviruses need to be investigated in patients with EKC.