Prevalence and genotypic characterization of human parvovirus B19 in hemophilia patients

Mohsen Keshavarz; Nahid Janati-Namin; Yaser Arjeini; Talat  Mokhtari-Azad; Farhad Rezaei

doi:10.18502/ijm.v14i4.10244

Mohsen Keshavarz The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Nahid Janati-Namin Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Yaser Arjeini Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
Talat Mokhtari-Azad Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Farhad Rezaei Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijm.v14i4.10244

Keywords: Human parvovirus B19; Hemophilia; Seroprevalence; Blood transfusion; Blood disease; Iran

Abstract

Background and Objectives: Parvovirus B19 (B19V) is usually transmitted through respiratory tract, but can also be received through blood transfusion. This study evaluated the seroprevalence, DNA existence, and circulating genotypes of B19V in hemophilia patients.

Materials and Methods: Serum samples of cases and controls were analyzed for B19V using ELISA and real-time PCR. Finally, obtained sequences were used for genotyping.

Results: Among cases, 3% were anti-B19V IgM positive and 47% were anti-B19V IgG positive and B19V DNA was detect- ed in 16% of them. However, among controls, 38% were anti-B19V IgG positive (P>0.05) and 5% were B19V DNA positive (P= 0.019). Also ~13% of cases were positive and all of controls were negative for IgG avidity test (P= 0.029). Viral load in case group was higher than control group (P = 0.037).

Conclusion: Since hemophilia patients receive large amounts of blood factors, prevalence of B19V in these patients might be higher than normal subjects.