First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

Abdelhamid Massik; Lahbib Hibaoui; Benboubker Moussa; Ghita Yahyaoui; Bouchra Oumokhtar; Mustapha Mahmoud

doi:10.18502/ijm.v14i4.10229

Abdelhamid Massik Laboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco
Lahbib Hibaoui Laboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco
Benboubker Moussa Human Pathologies, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco
Ghita Yahyaoui Laboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco
Bouchra Oumokhtar Human Pathologies, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco
Mustapha Mahmoud Laboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco

DOI: https://doi.org/10.18502/ijm.v14i4.10229

Keywords: Acinetobacter baumannii; Carbapenems; Resistance; Metallo-beta-lactamase

Abstract

Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Moroccan hospital.

Materials and Methods: Seventy carbapenem-resistant A. baumannii isolates from various samples were received at the mi- crobiology laboratory of the Hospital Center. Antibiotic susceptibility was tested by the diffusion disc method and molecular characterization of antimicrobial resistance was performed by PCR and sequencing.

Results: Carbapenemase genes were detected in our isolates: the OXA-51 gene and the ISbA1 sequence were detected in all isolates (100%), the OXA-23 and OXA-58 genes were detected in 82.85% and 10% of isolates respectively, MBL genes were dominated by VIM 39 isolates (55.7%), followed by GIM 26 isolates (37%), SIM 20 isolates (28.5%), IMP 8 isolates (11, 4%), NDM 3 isolates (4%) and for the first time in Morocco SPM with 4 isolates (5.7%).

Conclusion: The emergence of resistance of A. baumannii to carbapenems is a serious problem in our hospital which re- quires the establishment of a prevention strategy and strict respect for hygiene to minimize their dissemination.