Identification of the Most Common Allergens of Acer velutinum Pollen

Najmeh Sepahi; Soheila Alyasin; Zahra Kanannejad; Hossein Esmaeilzadeh; Farzaneh  Mohammadalizadeh Shirazi; Maryam Babaei; Shirin Farjadian

doi:10.18502/ijaai.v21i1.8620

Najmeh Sepahi Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Soheila Alyasin Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Zahra Kanannejad Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Hossein Esmaeilzadeh Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Farzaneh Mohammadalizadeh Shirazi Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Maryam Babaei Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Shirin Farjadian Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

DOI: https://doi.org/10.18502/ijaai.v21i1.8620

Keywords: Hypersensitivity; Immunoglobulin E; Pollen

Abstract

Pollens have been identified as potent inducers of allergic diseases worldwide. Acer velutinum (Persian maple) tree is an important source of allergic pollens in Iran. This study aimed to identify the immunoglobulin E (IgE)-reactive components of A. velutinum pollen extract in patients with maple allergy. We aimed to evaluate its allergenic components; using IgE in the serum of patients with maple allergy.

Twenty-two patients with a clinical history of reaction and a positive skin-prick test to maple pollen extract were included in this study. Identification of IgE-binding proteins in A. velutinum pollen extract was performed by immunoblotting using sera from sensitive patients.

A protein band with a molecular weight of around 70 kDa was the most IgE-reactive allergen in A. velutinum pollen extract detected by this method.

Identification of a protein with a molecular weight of about 70 kDa, as the most reactive allergen of A. velutinum pollen extract, can be considered as a potential allergen for designing diagnostic kits or as a target for immunotherapy of allergic patients with maple allergy.