Identification of Antibodies Against Neutrophil Surface Antigens  in Two Iranian Patients with Autoimmune Neutropenia

Behnaz Esmaeili; Behnaz Bayat; Mohamad Reza Fazlollahi; Leila Moradi; Arash Kalantari; Seyed Mohammad Kazem Nourbakhsh; Zahra pourpak

doi:10.18502/ijaai.v20i6.8029

Behnaz Esmaeili Immunology, Asthma and Allergy Research Institute (IAARI), Tehran University of Medical Sciences, Tehran, Iran
Behnaz Bayat Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany
Mohamad Reza Fazlollahi Immunology, Asthma and Allergy Research Institute (IAARI), Tehran University of Medical Sciences, Tehran, Iran
Leila Moradi Immunology, Asthma and Allergy Research Institute (IAARI), Tehran University of Medical Sciences, Tehran, Iran
Arash Kalantari Department of Pediatrics, Vali-e-Asr Hospital, Imam Khomeini Hospital Complexes, Tehran University of Medical Sciences, Tehran, Iran
Seyed Mohammad Kazem Nourbakhsh Department of Pediatrics, Vali-e-Asr Hospital, Imam Khomeini Hospital Complexes, Tehran University of Medical Sciences, Tehran, Iran
Zahra pourpak Immunology, Asthma and Allergy Research Institute (IAARI), Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijaai.v20i6.8029

Keywords: Agglutination Test; Autoimmunity; Indirect Immunofluorescence; Neutropenia

Abstract

Autoimmune neutropenia is a type of immune-mediated neutropenia, caused by antibody-induced neutrophil destruction. Here, we report two cases (a 3-year-old boy and a 9-year-old girl) with suspected autoimmune neutropenia. The presence of neutrophil antibodies in the sera of these two patients was investigated; using standard neutrophil antibody screening tests such as granulocyte immunofluorescence test (GIFT), granulocyte agglutination test (GAT), and lymphocyte immunofluorescence test (LIFT). A positive reactivity with two-panel cells was found in GIFT. No reactivities with panel cells were observed in GAT and LIFT. To the best of our knowledge, this is the first report for detecting the neutrophil reactive antibodies; using genotyped neutrophils in patients with autoimmune neutropenia in Iran. The final diagnosis of our patients was primary autoimmune neutropenia for the boy and autoimmune neutropenia associated with familial Mediterranean fever for the girl.