A Novel Nanodrug Suppresses Lung Cancer Growth and Metastasis in C57BL/6 Mouse Model by Altering CD8+ Cell Infiltration and Oxidative Stress

Sajjad  Shekarchian; Marzieh  Eghtedardoost; Hannaneh  Golshahi; Helia  Behrouzfar; Zahra  Fakhroueian; Roya  Yaraee

doi:10.18502/ijaai.v24i3.18682

Sajjad Shekarchian Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
Marzieh Eghtedardoost Arena Best Diagnostics Lab Ltd., Tehran, Iran
Hannaneh Golshahi Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Helia Behrouzfar Faculty of Veterinary Medicine, Science and Research Branch of Islamic Azad University, Tehran, Iran
Zahra Fakhroueian School of Chemical Engineering-Nanomedicine, College of Engineering and IPE, University of Tehran, Tehran, Iran
Roya Yaraee Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran

DOI: https://doi.org/10.18502/ijaai.v24i3.18682

Keywords: Animal model; Lung cancers; Nanomedicine; Nanoparticle; NSCLC; Zinc oxide

Abstract

Lung cancer is a leading cause of cancer deaths worldwide and new therapeutic approaches are needed. This study investigates the efficacy of a new zinc oxide-based nanomedicine in a mouse model of heterotopic lung cancer.

C57BL/6 mouse model with Lewis lung carcinoma (LL2) cells was used. The mice were treated with different doses of nanodrug, cisplatin, or phosphate-buffered saline. Tumor growth, metastasis, markers for oxidative stress, and immune responses, in particular the infiltration of CD8⁺ T cells, were examined.

The nanodrug significantly reduced tumor size, inhibited metastasis, and improved survival compared to the control group. Moreover, no significant toxic effect was observed in hematological, biochemical and histopathological analyses. Furthermore, the nanodrug altered the tumor microenvironment in favor of immune system activation by modulating the level of oxidative stress and increasing CD8⁺ cell infiltration.

The results show that this new nanomedicine may be a candidate for an effective treatment for lung cancer.