Evaluation of T-cell Function after Blood Transfusion in Patients Undergoing Coronary Artery Bypass Grafting

Nasim  Golafshani; Narges  Golafshani; Ali Reza  Mohseni; Mahboubeh  Mohseni; Shervin  Ziabakhsh-Tabari; Hossein  Asgarian-Omran; Mohsen  Tehrani; Ali Akbar  Pourfathollah; Ahmad  Najafi

doi:10.18502/ijaai.v24i3.18681

Nasim Golafshani Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Narges Golafshani Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Ali Reza Mohseni Department of Laboratory Sciences, School of Allied Sciences, Mazandaran University of Medical Sciences, Sari, Iran
Mahboubeh Mohseni Fatemeh Zahra Hospital of Sari, University of Medical Sciences, Mazandaran, Iran
Shervin Ziabakhsh-Tabari Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Hossein Asgarian-Omran Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Mohsen Tehrani Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Ali Akbar Pourfathollah Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Ahmad Najafi Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

DOI: https://doi.org/10.18502/ijaai.v24i3.18681

Keywords: Blood transfusion; Coronary artery bypass; Cytokines; Immunomodulation; T-lymphocytes; TH1 cells; TH2 cells TH1; TH2

Abstract

Blood transfusion is associated with increased mortality and morbidity. This study aimed to determine the effect of blood transfusion on T-helper 1 (T_H1), T_H2, and T_H17 function in patients undergoing coronary artery bypass grafting (CABG).

Two blood samples were obtained from patients undergoing CABG, before and 14 days after surgery. Production of interleukin (IL)-2, IL-4, interferon (IFN)-γ, IL-17A, and IL-10 by CD4⁺ T cells was measured using enzyme-linked immunosorbent assay (ELISA). mRNA expression of T-box expressed in T cells (T-bet), GATA binding protein 3 (GATA3), RAR-related orphan receptor-γ (ROR-γt), signal transducer and activator of transcription 3 (STAT3), STAT4, and STAT6 were measured using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).

mRNA expression of T-bet and STAT4 showed a significant decrease after blood transfusion. However, the concentration of IFN-γ in the culture supernatant showed no significant difference after blood transfusion. mRNA expression of GATA3 and STAT6 showed a significant decrease after blood transfusion. However, the concentration of IL-4 in the culture supernatant showed no significant difference after blood transfusion. mRNA expression of ROR-γt showed no significant decrease after blood transfusion; however, the expression of STAT3 and the concentration of IL-4 in the culture supernatant did significantly decrease following blood transfusion. IL-10 production increased significantly postoperatively.

Decreased T_H1, T_H2, and T_H17 signaling pathway activity and increased IL-10 concentration indicate an immunomodulatory effect on the immune system after blood transfusion.