The Immune Base Therapy of Pain with Magnesium Sulfate on the Trigger Axis of the TNF-α-TRAF6-NF-κB and Its Inhibitor (miR-146a-5p) in Rats

Gholam Reza  Bagheri; Javad  Poursamimi; Hadi  Ali-anvari; Sara Rashki  Ghalenoo; Hamid Reza  Ghaffari

doi:10.18502/ijaai.v24i2.18146

Gholam Reza Bagheri Department of Nutrition, School of Health, Zabol University of Medical Sciences, Zabol, Iran
Javad Poursamimi Department of Immunology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
Hadi Ali-anvari Department of Anesthesiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Sara Rashki Ghalenoo Department of Cardiology, Zabol University of Medical Sciences, Zabol, Iran
Hamid Reza Ghaffari Department of Basic Sciences, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran

DOI: https://doi.org/10.18502/ijaai.v24i2.18146

Keywords: Immunotherapy; Magnesium sulfate; MIRN146a microRNA, Paclitaxel; Pain; Rat; Tumor necrosis factor-alph

Abstract

Neuropathic pain can arise from injury or illness affecting the somatosensory system. It can also be triggered by cancer or chemotherapy drugs like paclitaxel. Researchers have indicated that magnesium sulfate may help in preventing neuropathy. This study aimed to investigate the effect of magnesium sulfate on paclitaxel-induced neuropathic pain by inhibiting the Tumor Necrosis Factor (TNF) Alpha - receptor-associated factor 6 - Nuclear factor kappa-light-chain-enhancer of activated B cells (TNF-α-TRAF6-NF-κB) axis.

Twenty-four male rats were divided into four groups: experiment group (E)-1, E2, E3, and the control group (Co). The experimental groups and the control group received paclitaxel at a dosage of 8 mg/kg every other day, totaling four injections over seven days. In addition, magnesium sulfate was administered daily in three doses of 300, 150, and 75 mg/kg, amounting to seven injections over the course of seven days. On the seventh day, peripheral blood samples were collected from the rats, and sera were used for the analysis of TNF-α serum levels and MicroRNA-146a-5p expression using ELISA and qRT-PCR methods, respectively.

The serum levels of TNF-α increased in the E1, E2, and E3 groups compared to the control group. However, there was a gradual decrease in the E1, E2 and E3 groups. The miR-146a-5p expression declined in the E1 group and increased in the E2 and E3 groups compared to the control group.

This study demonstrated that administering 300 and 150 mg of magnesium sulfate decreased TNF-α synthesis and reduced the function of the TNF-α-TRAF6-NF-κB axis during the initiation step.