Assessment of the Anti-cancer Effects of Camel Milk Exosomes (CMEXOs) on Murine Colorectal Cancer Cell Line (CT-26)

Samira Karbasi; Nafiseh Erfanian; Hamideh Dehghan; Asghar Zarban; Mohammad Hasan Namaei; Mohammad Yahya  Hanafi-Bojd; Saeed Nasseri

doi:10.18502/ijaai.v23i3.15641

Samira Karbasi Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Nafiseh Erfanian Student Research Committee, Department of Molecular Medicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
Hamideh Dehghan Student Research Committee, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
Asghar Zarban Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Mohammad Hasan Namaei Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Mohammad Yahya Hanafi-Bojd Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
Saeed Nasseri Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran

DOI: https://doi.org/10.18502/ijaai.v23i3.15641

Keywords: Camel milk; Colorectal cancer; Exosomes; IL-6; TNF-α

Abstract

Today, camel milk consumption in the Middle East is trendy because it is believed that it reduces the risk of cancer. Recently, studies have discovered that most of milk's beneficial effects are because of its nanoparticles, especially exosomes. The objective of the present research was to investigate the anti-cancer effects of camel milk exosomes (CMEXOs) in the murine colorectal cancer cell line (CT-26).

Our findings verified the existence of exosomes measuring approximately 114.1±3.4 nm in diameter. Through MTT and migration assays, we established that CMEXOs exhibit dose-dependent anti-proliferative and anti-migration effects on the CT-26 cell line. Furthermore, our study showed that treatment with CMEXOs led to a reduction in TNF-α and IL-6 gene expression in CT-26 cells.

While additional in vivo studies are required, our data demonstrate that CMEXOs have anti-proliferative and anti-migration effects on CT-26, possibly by influencing crucial genes within the inflammation pathway.