PD-1/PD-L1 Interaction Regulates BCL2, KI67, BAX, and CASP3, Altering Proliferation, Survival, and Apoptosis in Acute Myeloid Leukemia

Mojdeh Soltani; Mahshid Vosoughi; Mazdak Ganjalikhani-Hakemi; Hoorieh Shapoorian; Pezhman Beshkar; Nahid Eskandari; Behrooz Ghezelbash

doi:10.18502/ijaai.v22i5.13998

Mojdeh Soltani Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Mahshid Vosoughi Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Mazdak Ganjalikhani-Hakemi Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Hoorieh Shapoorian Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Pezhman Beshkar Department of Laboratory Sciences, School of Allied Medical Sciences, Shahrekord University of Medical Sciences, Shahrekord, Iran
Nahid Eskandari Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Behrooz Ghezelbash Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

DOI: https://doi.org/10.18502/ijaai.v22i5.13998

Keywords: Acute myeloid leukemia; Apoptosis; Cell proliferation; Programmed death-ligand 1

Abstract

Programmed death ligand‑1 (PD‑L1) is a pivotal inhibitory checkpoint ligand known to induce T-cell exhaustion via interaction with the programmed death‑1 (PD‑1) receptor. Beyond this, PD-L1’s intrinsic signaling pathways within cancer cells warrant further exploration. This study aims to elucidate the effect of PD-L1 stimulation on the proliferation, survival, and apoptosis of acute myeloid leukemia (AML) cell lines.

Two human AML cell lines, HL-60 and THP-1 were cultured and treated with phorbol 12-myristate 13-acetate (PMA) to induce PD-L1overexpression. Post-treatment PD-L1 expression was confirmed via flow cytometry. Subsequently, cell surface PD-L1 was stimulated using a recombinant PD-1, 24 hours post-PMA treatment. The expression alterations in pivotal genes including BCL2, MKI67, BAX, and CASP3 were monitored using quantitative real-time polymerase chain reaction 24 and 48 hours post-treatment. Additionally, annexin-V through flow cytometry.

Findings reveal that PD-L1 stimulation augments AML cell proliferation and survival by enhancing MKI67 and BCL2 expressions while concurrently inhibiting cell apoptosis due to decreased BAX and CASP3 expression following PD-L1 stimulation. Notably, stimulated cells expressed exhibited reduced annexin-V compared to control cells.

This study underscores that PD-L1 stimulation fosters AML cell proliferation and survival while impeding cell apoptosis. The results hold potential implications for targeting PD-L1 in AML treatment strategies.