Association of Gene Polymorphisms in CXC Chemokine Receptor 5  with Rheumatoid Arthritis Susceptibility

Zhenzhen Su; Lin Chen; Qian Niu; Bin Yang; Zhuochun Huang

doi:10.18502/ijaai.v21i5.11041

Zhenzhen Su Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan, China
Lin Chen Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China
Qian Niu Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan, China
Bin Yang Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan, China
Zhuochun Huang Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan, China

DOI: https://doi.org/10.18502/ijaai.v21i5.11041

Keywords: CXC chemokine receptor 5; Disease susceptibility; Rheumatoid arthritis; Single nucleotide polymorphism

Abstract

Rheumatoid arthritis (RA) is caused by complicated interactions between genes and the environment. CXC chemokine receptor 5 (CXCR5) is required for B and T follicular helper cell migration and humoral immunity generation. Therefore, this study aimed to assess whether polymorphisms of the CXCR5 gene are implicated in RA development and progression.

This case-control study enrolled 285 RA patients and 291 healthy controls. The polymerase chain reaction-ligase detection reaction method was used to genotype rs630923, rs497916, rs3922, and rs676925 in the CXCR5 gene. Epidemiological, clinical, and laboratory data were collected retrospectively.

The rs630923 A allele was associated with a higher risk of RA (AOR [adjusted odds ratio]=2.00, 95% confidence interval [CI] =1.14–3.53). However, in the RA group, the frequency of the rs497916 T allele was lower (AOR=0.69, 95% CI=0.51–0.93). Regarding rs3922, AG+GG genotype carriers were at a significantly lower risk for RA than AA genotype carriers (AOR=0.70, 95% CI=0.49–0.99). In the RA group, we found that the different genotypes were significantly associated with specific laboratory values, including rheumatoid factor, total bilirubin, total cholesterol, low-density lipoprotein cholesterol, and alkaline phosphatase.

This is the first report indicating that CXCR5 polymorphisms were associated with RA susceptibility. These findings lead to a rising possibility of identifying RA-susceptible individuals based on genetic markers.