Chemical compositions and antifungal activities of Satureja macrosiphon against Candida and Aspergillus species

Marjan   Motamedi; Mohammad Jamal   Saharkhiz; Keyvan   Pakshir; Sara   Amini Akbarabadi; Marzieh  Alikhani Khordshami; Fatemeh  Asadian; Zahra   Zareshahrabadi; Kamiar  Zomorodian

doi:10.18502/cmm.5.4.2162

Marjan Motamedi
Mohammad Jamal Saharkhiz
Keyvan Pakshir
Sara Amini Akbarabadi
Marzieh Alikhani Khordshami
Fatemeh Asadian
Zahra Zareshahrabadi
Kamiar Zomorodian

DOI: https://doi.org/10.18502/cmm.5.4.2162

Keywords: Essential oil, MTT assay, Satureja macrosiphon, XTT assay

Abstract

Background and Purpose: Despite the various applications of Satureja species, there are limited data in this domain. Regarding this, the present study was conducted to investigate the essential oil (EO) biological activity of S. macrosiphon species in Iran.
Materials and Methods: The EO of S. macrosiphon flowers was obtained by hydrodistillation. Chemical compositions of the EO were analyzed using gas chromatography-mass spectrometry. In addition, minimum inhibitory concentrations (MIC) were measured by means of the broth microdilution method. The estimation of antibiofilm and cytotoxic activities was also accomplished using the tetrazolium salt and MTT assays, respectively.
Results: A total of 26 components were identified in the EO with linalool as the main constituent (28.46%). A MIC range value of 0.25-8 μL/mL was obtained against all of the tested fungi. The EO inhibited the biofilm development of the Candida tested strains at a concentration of 4-8 μL/mL. Cytotoxicity (IC50) of EO against the HeLa cell was greater than the MIC concentration (6.49 μL/mL).
Conclusion: Based on the findings, it was concluded that the EO of S. macrosiphon has the potential for further use as an antifungal agent.