Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea

Ladan   Nazemi; Seyed Jamal  Hashemi1; Roshanak Daie Ghazvini; Mina  Saeedi; Sadegh  Khodavaisy; Aleksandra  Barac; Mona   Modiri; Maryam   Akbari Dana; Zahra  Zare shahrabadi; Sassan   Rezaie

doi:10.18502/cmm.5.3.1745

Ladan Nazemi
Seyed Jamal Hashemi1
Roshanak Daie Ghazvini
Mina Saeedi
Sadegh Khodavaisy
Aleksandra Barac
Mona Modiri
Maryam Akbari Dana
Zahra Zare shahrabadi
Sassan Rezaie

DOI: https://doi.org/10.18502/cmm.5.3.1745

Keywords: Aspergillus fumigatus, CgrA gene, Cyp51A gene, Kombucha

Abstract

Background and Purpose: Aspergillus fumigatus is one of the most common opportunistic fungus, which causes infection in immunocompromised and neutropenic patients. The current guidelines recommend voriconazole as the initial therapeutic and prophylactic agent for almost all cases, especially in patients with organ transplants, which leads to increased medication resistance in A. fumigatus. The aim of the present study was to evaluate the antifungal activity and effect of kombucha as a natural compound on A. fumigatus growth, as well as on the expression of cgrA and cyp51A genes.

Materials and Methods: A panel of 15 A. fumigatus strains with two quality controls of CM237 and CM2627 as susceptible and resistant strains were obtained from Tehran Medical Mycology Laboratory, Tehran,Iran(TMML).Antifungal susceptibility testing assay was performed according to the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document. Moreover, the mycelial dry weight of the fungus was calculated before and after being treated with kombucha. In addition, the quantitative changes in the expression of cgrA and cyp51A genes were analyzed by real-time polymerase chain reaction (real-time PCR) technique.

Results: In the present study, the minimum inhibitory concentration ranges of kombucha were measured at 6,170 and 12,300 μg/mL for ten A. fumigatus azole-susceptible strains and 24,700 μg/mL for five A. fumigatus resistant strains. Moreover, changes in mycelial dry weight under kombucha treatment conditions underwent a significant reduction (P≤0.05). A coordinate down-regulation of expression in cgrA and cyp51A genes was observed in all azole-susceptible and -resistant A. fumigatus strains, after treating the fungus with different concentrations of kombucha (P≤0.05).

Conclusion: According to the obtained results, kombucha as a natural antioxidant , can exert inhibitory effects against the growth and expression of some genes in A. fumigatusstrains.