Evaluating the MicroRNA Expression of IL-35 and IL-37 in  Helicobacter pylori-infected Patients with Gastritis and Gastric Ulcer

Zahra Ahmadnia; Mohammad Ranaee; Rouzbeh  Mohammadi Abandansari; Nader Bagheri; Hedayatollah Shirzad

doi:10.18502/ijaai.v21i1.8609

Zahra Ahmadnia Cellular and Molecular Research Center, Basic Health Sciences Institute, School of Medicine,
Mohammad Ranaee Clinical Research Development Unit of Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran
Rouzbeh Mohammadi Abandansari Clinical Research Development Unit of Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran
Nader Bagheri Cellular and Molecular Research Center, Basic Health Sciences Institute, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Hedayatollah Shirzad Cellular and Molecular Research Center, Basic Health Sciences Institute, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

DOI: https://doi.org/10.18502/ijaai.v21i1.8609

Keywords: Gastritis; Helicobacter pylori; Human interleukin-35; Human IL-37 protein; Stomach ulcer

Abstract

Interleukin (IL)-35 and IL-37 are two anti-inflammatory cytokines. IL-35 inhibits the development of T-effector cells such as Th1, and Th17; while increasing regulatory T cells (Tregs). IL-37 causes the suppression of inflammatory cytokines. Regarding the positive impact of Helicobacter pylori (H. pylori) infection on inflammation and considering the anti-inflammatory effects of IL-35 and IL-37, this study aimed to evaluate the expression of these two cytokines in H. pylori-infected patients with gastrointestinal problems.

The case group consisted of H. pylori-infected individuals with gastric ulcer and/or gastritis (n=50) and the control group consisted of cases with gastric ulcer and/or gastritis non-H. pylori-infected (n=50). Sampling and classification of patients were based on pathology findings. A real-time polymerase chain reaction was performed for evaluating the IL-35 and IL-37 expression levels.

h.pylori-infected gastritis patients showed lower expression of IL-35 and IL-37 than the non-infected group. There was a significant difference between the expression levels of IL-35 and IL-37 in patients with gastric ulcers and/or gastritis who were infected and non-infected by H. pylori. There were no significant differences in the expression level of IL-35 and IL-37 in H. pylori-infected patients with gastric ulcer or gastritis.

Interleukins 37 and 35 were less expressed in patients with H. pylori-infection. In differentiation between patients with gastrointestinal symptoms who have H. pylori infection or with similar symptoms who do not have H. pylori-infection, mentioned interleukins can be used as diagnostic markers.