Journal of Zabol Medical School

Investigating the Effect of Lippia Citriodora Leaf Hydro-Ethanolic Extract on Two Prostate Cancer Cell Lines PC-3 and LNCaP Alone and in Combination with Docetaxel

Mehdi Fazeli — Sun, 28 Jan 2024 03:56:55 +0000

Background: Prostate cancer is the most common cancer in men. Docetaxel is the most important drug for prostate cancer, but drug resistance reduces its effectiveness. One of the important strategies to increase the drug efficacy is the use of combination therapy. This study investigated toxic effect of Lippia citriodora leaf extract on two prostate cancer cell lines and its effect on docetaxel cytotoxicity.

Methods: Cytotoxicity of Lippia citriodora leaf extract was evaluated individually or in combination with docetaxel. Cells were cultured in 96-well plates. After treating cells with different concentrations of extracts and drugs, their survival was measured by MTT assay.

Results: Lippia citriodora leaf extract showed a highly toxic effect on prostate cancer cells. The extract at the highest concentration, decreased the percentage of viable cells to 18.01 ± 1.37 in PC-3 cells and to 20.80 ± 1.06 in LNCaP cells. The IC50 value of the extract was 78.14 mg/ml for PC-3 cells and 88.76 mg/ml for LNCaP cells. In addition, the extract increased the cytotoxicity of docetaxel. In the maximum increasing effect, the percentage of survival in PC-3 and LNCaP cells decreased from 50.87 ± 2.46 to 26.48 ± 1.12 and from 54.13 ± 1.83 to
28.84 ± 1.69, respectively.

Conclusion: Lippia citriodora leaf extract had a remarkable toxic effect on prostate cancer cells in vitro and increased docetaxel cytotoxicity. The antitumor effects of the extract can be further evaluated in vivo and clinical trials.

Comparison of the Effect of Two Drug Combinations: Ketamine-Midazolam -Sufentanil with Etomidate-Sufentanil on Changes in Heart Rate and Blood Pressure After Laryngoscopy and Endotracheal Intubation

Sun, 28 Jan 2024 04:01:24 +0000

Background: Due to the high prevalence of complications during and after laryngoscopy, especially hemodynamic disturbances, and the absence of a suitable solution for preventing these complications, this study was conducted to compare the effect of “Ketamine-Midazolam-Sufentanil” with “Etomidate-Sufentanil” combinations on changes in heart rate and blood pressure after laryngoscopy and endotracheal intubation.

Methods: In this controlled, triple-blind randomized clinical trial, 96 laryngoscopy candidate patients were divided into three groups of 32 members and were given “Ketamine-Midazolam-Sufentanil” (KMS), “Etomidate-Sufentanil” (ES) and” Etomidate- Normal Saline” (NS) respectively before laryngoscopy. Hemodynamic parameter changes at baseline and 1,3,5 and 10 minutes after laryngoscopy, were measured and compared between the 3 groups.

Results: During the study, the 3 groups KMS, ES, and NS had 0%, 15.6% and, 3.1% bradycardia respectively (p value = 0.024). Changes in systolic blood pressure were significantly different in the 3 groups (p value = 0.043). However, the incidence of tachycardia, hypertension, and hypotension were not significantly different among the 3 groups.

Conclusion: Results of this study showed that using the “Ketamine-Midazolam-Sufentanil” combination before laryngoscopy can reduce hemodynamic disturbances during and after laryngoscopy. Patients who receive this drug combination seem to have more desirable hemodynamic stability during and after laryngoscopy.

Evaluation of 5-Year Survival Rate of Patients with Multiple Myeloma Undergoing Bone Marrow Transplantation: A Descriptive Study

Ali Nemati, Elahenaz Parsi Mood, Shirin Raghi, Vahid Moazed — Sun, 28 Jan 2024 04:05:44 +0000

Background: Multiple myeloma is a progressive and high mortality neoplasm, and the prevalence increases with aging. The main treatment for new cases aged less than 65 years is high-dose chemotherapy with autologous bone marrow transplantation. This study aims to determine the five-year survival rate of patients with multiple myeloma.

Methods: In this descriptive-analytical study, 97 patients with multiple myeloma who underwent bone marrow transplantation between 2011 and 2016 were investigated. Then, information related to gender, age at the time of transplantation, the time interval between diagnosis and transplantation, cases of death and, date of death of the studied patients were collected, and the survival of the patients from the date of diagnosis to the end of the study period (five years) was calculated. Data were analyzed using Cox proportional and chi-square tests.

Results: Five-year survival of patients was 59.8%. The median overall survival of patients was 54.22 months, which shows that age (p value = 0.003), gender
(p value = 0.591), and the time delay between diagnosis and bone marrow transplantation (p value = 0.005) all affect patient survival. There was a significant relationship between the patient's age and the time interval between diagnosis and transplantation with the five-year survival of the patients.

Conclusion: As a result, bone marrow transplantation provides a higher survival rate. Also, the effect of the patient‘s age and time interval between diagnosis and transplantation, early diagnosis of the disease and, earlier bone marrow transplantation can bring longer survival for patients.

Evaluation of the Effect of Regular Administration of Coagulant Factors on the Joint Pain Level and Dimensions of the Quality of Life of the Patients Suffering from Some Bleeding Disorders (BDs)

Sun, 28 Jan 2024 04:10:15 +0000

Background: Blood coagulation is a process that prevents blood loss. Lack of coagulation factors (inherited or acquired) disrupts this path. The prevalence of coagulant deficiency including VIII and XIII factors in Sistan and Baluchistan province is higher than in other parts of Iran. This study aimed to investigate the condition of patients suffering from some bleeding disorders (BDs) in terms of regular coagulant takes and their effects on quality of life.

Methods: Our cross-sectional study was descriptive-analytical. Thirty-five BD patients suffering the blood coagulant deficiency enrolled, and were sorted into 5 groups.

Results: The mean (±SD) age in the hemophilia A and B groups were
(16.25 ± 8.15), and (8.14 ± 3.9), and other coagulation factor deficiencies including VWF, XIII, and IX were (15.1 ± 7.8), (15.5 ± 2.4), and IX (19.0 ± 11.5) respectively. The quality of life in group A (81.3 ± 14.9) compared to groups B (50 ± 0.25) and IX (47.5 ± 24.1) had a significant difference (p value < 0.05). In addition, there was a significant difference in group XIII (79.2 ± 29.2) compared to groups B (50 ± 0.25) and IX (47.5 ± 24.1) (p value < 0.05). Generally, the quality of life was higher in women (10 people) (72.5 ± 26.2) than in men
(26 people) (61.1 ± 24.5). Furthermore, the Visual analog scale for pain in group A (1.3 ± 0.4) had a significant difference compared to group B (3.5 ± 0.43), IX (7.8 ± 0.9), XIII (0.4 ± 4.3) and VWF (3.2 ± 0.38) (p value < 0.05).

Conclusion: In sum, patients with hemophilia A and coagulation factor XIII deficiency had low levels of joint pain and a high level of quality of life dimensions.

Investigating the Relationship between Happiness and Environmental Attitude among Adults in Yazd City

Mozhgan Mojkar, Mohammad Ali Marvoti, Abbas Rahimi, Abulqasem Pourreza — Sun, 28 Jan 2024 04:14:43 +0000

Background: Happiness, as one of the most important psychological needs of humans, may have an impact on the environmental behavior of people due to its major effects on the formation of human personality. Therefore, the purpose of this study is to determine the relationship between happiness and environmental attitudes in adults of Yazd City in 2019.

Methods: This cross-sectional descriptive study was conducted on 230 people over 18 years of age in Yazd, and the sampling method in this study is simple random classification sampling and descriptive statistics (mean and standard deviation), as well as Pearson correlation coefficient statistics and the Kolmogorov-Smirnov test, and Variance was used.

Results: The results of this study showed that there is a significant relationship between the level of happiness and the type of attitude of people, the type of attitude and residential area, the level of happiness and education of the people of Yazd city regarding the relationship between happiness and environmental attitude (p value ≤ 0.001). Finally, the variable of education and residential area affects the attitude toward the environment, and the results showed that these variables have a significant relationship.

Conclusion: The most important conclusion of this research is the huge impact of the residential area on happiness and environmental parameters, which shows the influence of humans on the residential environment. This study showed that happiness is related to the environment.

Biological and Psychological Investigation of Sexual Desire Disorder in Infertile Men Referred to Avicenna Infertility Treatment Center

Nasser Amirjanti, Behzad Ghorbani, Zahra Sehat, Saeed Razi — Sun, 28 Jan 2024 04:20:07 +0000

Background: Decreased sexual desire in infertile men, which sometimes leads to infertility, can open the way to choose between drug or psychotherapy treatments for these patients, compared to psychological causes. Therefore, the present study was conducted to compare biological and psychological evaluation in men with and without sexual desire disorder in infertile patients referred to Avicenna Infertility Treatment Center.

Methods: A descriptive-analytical study was conducted on infertile men. The studied sample was estimated to be 128 people using G-power software, taking into account the first type error of 0.05, statistical power of 0.8, and effect size of 0.5, from the eligible infertile men by random sampling were chosen. Halbert's Sexual Desire Questionnaire (HISD) was used to measure sexual desire, and Zanke's standard questionnaire was used to measure anxiety.

Results: In this study, 128 men were examined. Among these people, 39.8% had the cause of male infertility and 11.7% had the cause of female infertility. 3.1% of the people had cryptorchidism, 32% had a history of varicocele, and 7% mentioned infection. Zonk score classification in the participants in the study: 73.33 ± 21.62, normal anxiety, 60.28 ± 13.62, mild anxiety, 50.50 ± 22.63, severe anxiety, and 44.50 ± 16.54, very severe anxiety they had. Among the investigated variables, the level of anxiety and depression is more related to smoking, Cryptorchidism, no history of varicocele, or history of infection.

Conclusion: Infertile men, in addition to reducing their cognitive well-being, suffer a heavy psychological burden that can cause dysfunction and a decrease in sexual desire.