Journal of Shahid Sadoughi University of Medical Sciences

Protecting Public Health Rights in Yazd Province: Prioritizing Challenges and Solutions

Saeed Hosseini, Vali Bahrevar, Zahra Naqibi Nodoushan, Seyed Masood Mousavi — Sun, 29 Dec 2024 10:29:52 +0000

Introduction: The right to health is one of the fundamental human rights, which includes access to healthcare services, suitable living conditions, and a healthy environment. In Iran, this right is clearly stated in the Constitution and key documents, and the government is responsible for providing these services to all individuals. In Yazd Province, a committee for the protection of people's rights in health has been established to identify the challenges and implementation problems in this area. This research has been conducted qualitatively and quantitatively to identify and prioritize issues related to individuals’ health rights in Yazd Province.

Examining Effective Strategies for Improving Sexual Satisfaction in Women during Pregnancy and Postpartum: A Review Study

Farzaneh Rahimi, Mahshid Moenimehr, Fariba Slam — Sun, 29 Dec 2024 10:31:56 +0000

Introduction: The period of pregnancy and postpartum is characterized by physical and psychological changes in women, which can significantly contribute to the onset or exacerbation of sexual disorders. Various nursing and midwifery interventions have been used as alternative therapeutic methods to improve sexual satisfaction in women during pregnancy and after childbirth, and this study aims to review and summarize these interventions.

Methods: A total of 40 studies (13 Persian articles and 27 Latin articles) have been examined. Nine distinct approaches have been utilized to enhance sexual satisfaction during pregnancy (17 studies) and following childbirth (23 studies). The influence of educational techniques for mothers across various areas (14 studies) during pregnancy, along with group and sexual counseling (10 studies) and group and sexual counseling (10 studies) and instruction on pelvic and leg exercises (9 studies) in the postpartum phase, appears to be more prevalent than other approaches in the research. is more frequent than other methods in the studies. Researchers have conducted an investigation. .

Results: Uncoordinated movement was noted in 75% of mice following prolonged acetaminophen use (p<0.01) while pretreatment with Stevia entirely counteracted the effects of acetaminophen. Acetaminophen was significantly (p<0.01) increased serum AST and ALT and Sativa extract dose dependently mitigated the effects of acetaminophen. Pretreatment with both doses of stevia leads to a decrease in symptoms of liver necrosis caused by chronic acetaminophen use.

Conclusion: The approach of educating mothers in different areas, including sexual counseling and pelvic and Kegel exercise, is effective during pregnancy and post-delivery by midwifery professionals in the centers due to simplicity, accessibility, and affordability. Offering services and clinics have been utilized to enhance sexual satisfaction and improve the quality of women's sexual experiences, helping them better adjust to their pregnancy and postpartum periods.

Frequency and Causes of Dental Complaints in the Yazd Medical Council Organization between 2017 and 2022

Yasaman Sabaghzadegan, Sina Amini-Bahabadi, Fatemeh Owlia — Sun, 29 Dec 2024 10:33:42 +0000

Introduction: Currently, even with the extensive and advanced resources available in dentistry, patient dissatisfaction and complaints have increasingly become more prevalent. To acquire accurate and practical data for enhancing dental services’ quality and reducing the expenses linked to complaints, research must be performed to explore dental grievances. This research sought to identify the rate and reasons behind complaints made against dentists to the healthcare system from 2017 to 2022. Methods: This cross-sectional research entailed gathering existing data and examining complaint records from dentists at the Yazd Medical Council Organization. The information was gathered through observation and by filling out applicable checklists. Subsequently, thedata underwent analysis through SPSS version 16, utilizing with a chi-square test and Spearman correlation. The threshold for statistical significance was established at p≤ 0.05.

Results: According to the results obtained, the majority of complaints were directed towards general dentists, especially males. Over fifty percent of the individuals who filed complaints were female. The most frequent types of dental issues were associated with surgery, endodontic, and prosthetic care. In 52.9 percent of the cases, dentists were deemed guilty, primarily due to complaints related to malpractice.

Conclusion: Dental practitioners must increase their knowledge regarding case selection and dental management to improve their practice.

Immunohistochemical Study of Expression of Cyclynd1 Expression in Invasive and Non-invasive Odontogenic Cysts

Najmeh Jafari, Seyed Hosein Tabatabaei, Raheleh Karimi — Sun, 29 Dec 2024 10:35:34 +0000

Introduction: A radicular cyst is an inflammatory cyst arising from the remnants of epithelial malassez, while the Dentigerus cyst is linked to the impacted crown. The odontogenic Keratocyst is a developmental cyst that exhibits suggesting invasive behavior and a propensity to adjacent tissues, potentially due to the proliferation of the epithelial lining or the enzymatic composition of the cyst wall. CyclynD1 is a protein in the Rb pathway that regulates the shift from G1 to S phase. This protein can enable the cell to bypass cell cycle checkpoints and is crucial in lesion development. Considering the varied outcomes and limited research on the expression of the aforementioned marker in odontogenic cysts ، the aim of this study was to compare odontogenic keratocysts (OKC) with two types of non-invasive inflammatory and growth-developmental cysts.

Methods: Following a review of the patient records from the Pathology Department of Dental School of Yazd for all jaw cysts، 15 blocks from each of the three cyst types with adequate tissue were chosen for immunohistochemical staining using the Cyclin D1 marker. The stained cell count under a light microscope at x400 magnification was performed and the findings were analyzed using SPSS16 software utilizing Mann-Whitney and Kruskal-Wallis tests.

Results: There was no statistically significant difference observed among the three cysts regarding epithelial coverage (p = 0.226) and connective tissue (P = 0.578).

Conclusion: Based on the findings of this study، the high expression of this marker in odontogenic Keratocyst may signify its involvement in the pathogenesis and progression of this cyst in contrast to other cysts.

The effect of eight weeks of high intensity interval training (HIIT) on the content of mitochondrial fission and fusion proteins in the left ventricle of old rats

Sun, 29 Dec 2024 10:40:06 +0000

Introduction: As individual grow older, the likelihood of developing cardiovascular diseases increases significantly. Currently, high-intensity interval training (HIIT) is regarded as a strategy for exercise to improve heart disease. Consequently, the aim of this research was to investigate the impact of eight weeks of HIIT on the levels of PINK1 and PARKIN proteins in the left ventricle of aged rats.

Methods: The present research was of an experimental type and involved twelve male rats, each 20 months old, with an average weight of 400 ± 30 grams. The rats were randomly divided into HIIT and control groups (each group had 6 rats).

The HIIT program consisted of high-intensity bouts of 85-95% of maximum speed (m/min) and active rest periods with an intensity of 40-55% of maximum speed (m/min) during each weekly session. Following a 48-hour period post the most recent training session, the rats were subjected to anaesthesia through the administration of Ketamine and Xylazine. Subsequently, the left ventricle of the heart was excised, and the various parameters were measured utilizing the western blot laboratory technique. Data were analysed using the Shapiro-Wilk test and independent t-test conducted within SPSS16. A significance threshold of P≥0.05 was considered for this analysis.

Results: Eight weeks of HIIT caused a significant increase in PINK1 (P=0.02) and PARKIN (P=0.004) protein levels in the left ventricle of aged rats, in comparison to the control group.

Conclusion: It seems that HIIT may influence the quality of mitochondria within the left ventricle of the hearts of aged rats, indicating potential implications for health-related applications.

Isolation of viable follicles from cryopreserved human ovarian tissue

Sun, 29 Dec 2024 10:42:55 +0000

Introduction: Ovarian tissue freezing is the most effective method to maintain fertility for immature girls and women diagnosed with cancer. Nonetheless, because of the chance that malignant cells might reappearing following tissue transplantation, it is crucial to isolate the follicles from the frozen-thawed ovarian tissue of these individuals and employ them in the process of in vitro maturation process or artificial ovarian framework. This study aimed to assess the application of neutral red (NR) vital dye alongside collagenase IA for effectively isolating viable follicles from the vitrified human ovarian tissue samples.

Methods: Two categories existed: the category with NR and the group without NR. Chopped (0.5×0.5 mm) strips of vitrified-warmed ovarian tissue from 10 transsexual individuals were placed into two falcon tubes with HTCM and Collagenase IA (1mg/ml). Neutral red (NR) was introduced to one of the falcons. Follicles were then isolated mechanically. The morphology, size, and viability of the follicles were assessed. The condition of the follicles was evaluated using fluorescent staining methods involving Calcein-AM and Ethidium homodimer-I. The t-test method was used to evaluate the data.

Results: The number of isolated follicles with Neutral Red (46.50±25/02) exceeded those without NR (6.6±5.58; P < 0/0001). Additionally, according to the morphological studies, a majority of the isolated follicles from the transsexual ovarian cortex were primordial (77.4%), and primary (21.12%) follicles, with only a small number of secondary follicles (1.4%) identified in these tissues. Live/dead staining verified the viability of isolated follicles by displaying a green hue.

Conclusion: The finding indicates that combining collagenase I with the vital dye Neutral red significantly facilitates the of follicles from dense human ovarian tissue.

Impact of Exam Stress on Oral Hygiene and Periodontal Indices of Yazd Dental Students in 2024

Fahimeh Rashidi Maybodi, Mehrdad Homaei Mehr, Maryam Sabet — Sun, 29 Dec 2024 10:44:39 +0000

Introduction: Exams can be a potential cause of increasing students' academic stress. The current study examined the impact of exam-related stress on the oral health practices and periodontal measures of Yazd dental students in 2024.

Methods: In this cross-sectional study, a census was conducted on 42 fourth-year students. Following the acquisition of consent, two weeks prior and during the final week of the exams, measurements of the plaque index (PI), gingival index (GI), and stress levels were taken using the Gadzella questionnaire for comparison. Furthermore, the connection among gender, living apart from the family, and dormitory life concerning stress levels was explored. The students’ oral health habits of the students were also assessed via self-reporting. No guidance or hygiene instructions were given to the participants, and if there was any alternation in the frequency of brushing or flossing, the sample was excluded. Data analysis was conducted using SPSS22 software along with and paired T, Wilcoxon and Pearson tests.

Results: Gender, living with the family or independently, and living in a dormitory or a private home had no impact on students’ stress levels. The alternations in the PI and GI indices as well as academic stress changes were notable (P-value< 0.001). No significant correlation was detected between PI and GI concerning the frequently of brushing or flossing (P-value >0.05).

Conclusion: Taking into account the rise among students during exam periods along with the absents of a link between these changes and their overall oral hygiene habits, it seems that the stress from exams adversely affects oral health.

Investigation of Kidney Histopathology in Rats Receiving Methamphetamine Simultaneously with Berberine Hydrochloride and Aerobic Exercise

Sun, 29 Dec 2024 10:47:45 +0000

Introduction: Given the widespread issue of methamphetamine addiction and its damaging effects, coupled with the crucial role of the kidneys in maintaining bodily equilibrium, we chose to undertake this study.

Methods: Forty rats were randomly divided into five equal groups: control, methamphetamine, methamphetamine with exercise, methamphetamine with berberine hydrochloride, and methamphetamine with both exercise and berberine hydrochloride. The administration of methamphetamine at a dose of 15 mg/kg intraperitoneally for a duration of 4 days every 12 hours and berberine hydrochloride 100 mg/kg intraperitoneally for a duration of 4 weeks daily has been conducted. Aerobic exercise was performed in the form of running on a rodent treadmill for 8 weeks with moderate intensity. Twenty-four hours following the final exercise and administration of berberine hydrochloride, kidney tissue was extracted and examined under a light microscope, in accordance with the ethics committee's guidelines.

Results: In the methamphetamine group, the glomerulus diameter decreased, hyperemia and tissue disorganization occurred, and the cells of the proximal and distal convoluted tubules were atrophied. In the methamphetamine group, with exercise, there was a decrease in diameter in some glomeruli and changes in hyperemia and an increase in the diameter of blood vessels. In the group treated with methamphetamine and berberine hydrochloride, there was a noted shrinkage of glomeruli and an increase in urinary space. In the group receiving methamphetamine, exercise, and berberine hydrochloride, the quantity and size of glomeruli as well as the spaces between them, were normal and without irregularities.

Conclusion: This research demonstrated that both aerobic exercise and berberine hydrochloride can mitigate the damaging histopathological impacts of methamphetamine on the kidney, indicating that their simultaneous use may offer enhanced protective benefits. effect