Study of Polymorphism Rs4612666 in NLRP3 Gene in Patients with Type 2 Diabetes in East Azerbaijan

Fatemeh  Hosseinpour Shordarag; Hossien Soltanzade; Manochehr Ghojaie

doi:10.18502/ssu.v29i12.8976

Fatemeh Hosseinpour Shordarag Master Student of Genetics, Islamic Azad University, Bonab Branch, Bonab, Iran.
Hossien Soltanzade Islamic Azad University, Bonab Branch, Maragheh School of Medical Sciences, Maragheh, Iran.
Manochehr Ghojaie Islamic Azad University, Bonab Branch, Maragheh School of Medical Sciences, Maragheh, Iran.

DOI: https://doi.org/10.18502/ssu.v29i12.8976

Keywords: polymorphism rs4612666, Type 2 diabetes, NLRp3.

Abstract

Introduction: Type 2 diabetes is a common multifactorial disease. Studies have shown that the NLRP3 gene plays an important role in insulin resistance. The aim of this study was to investigate the association of rs4612666 polymorphisms of NLRp3 gene with type 2 diabetes in the population of East Azarbaijan province.

Methods: In this case-control study, blood samples from 110 patients and 110 healthy individuals as control group were collected. Following to extract of DNA from all samples and the quality assay using electrophoresis, the samples with their specific primers were examined by PCR and electrophoresis. Genotyping of individuals for rs4612666 was performed using PCR-RFLP method. Data were analyzed by SPSS software version 21 by descriptive and Chi-square test .The significance level was considered to be less than 0.05

Results: Analysis of the results showed a statistically significant difference (p <0.05) in the frequency of TT, CC and TC genotypes related to rs4612666 polymorphism at NLRP3 gene position (P = 0.000). The percentage of T allele in healthy and unhealthy individuals was 65.9% and 45.9%, respectively, and the percentage of C allele in healthy and unhealthy patients was 34.1% and 54.1%, respectively.

Conclusion: The results of this study suggest that there may be a link between an increase in the C allele and diabetes, although extensive studies are needed to confirm these results.