Effect of 4 weeks of High-Intensity Interval Training on P53 and Caspase-3 Proteins Content in the Heart Muscle Tissue of Rats with Type 1 Diabetes

Masoud Jokar; Mohammad  Sherafati Moghadam

doi:10.18502/ssu.v29i11.8502

Masoud Jokar Department Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran.
Mohammad Sherafati Moghadam Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran

DOI: https://doi.org/10.18502/ssu.v29i11.8502

Keywords: Apoptosis, High intensity interval training, Protein Caspase-3, Protein P53, Strepotozocin, Type 1 Diabetes.

Abstract

Introduction: Type 1 diabetes can lead to muscle apoptosis and a defect in the structure of the heart tissue. One of the main pathways of apoptosis is the pathway activated by the P53 and Caspase-3 proteins. Therefore, the aim of this study was to investigate the effect of 4 weeks of high intensity interval training on the content of P53 and Caspase-3 proteins in heart muscle tissue of Sprague-Dawley rats with type 1 diabetic rats.

Methods: In this experimental study, twelve 2-month-old Sprague-Dawley male rats with mean weight of 300±20 gr were selected and after induction of diabetes through streptozotocin solution, randomly divided into 2 groups: experimental (n= 6) and control (n= 6). The training program consisted of 5 stages of 4-minute with an intensity of 85 to 95% of the maximum speed and 3-minute active rest periods with an intensity of 50 to 60% of the maximum speed. Proteins were measured by Western-Blot in vitro. SPSS software version 16 and independent t-test were used to analyze the data.

Results: High intensity interval training resulted in a significant reduction in the P53 protein content of the training group compared to the control group (P=0.003). However, there was no significant change in the content of Caspase-3 in the activated form (P=0.71) and the initial form (P=0.15) in the training group compared to the control group.

Conclusion: High intensity interval training, by reducing the content of the P53 protein and preventing the activation of the caspase-3 protein, may have inactivated the apoptosis pathway of these two proteins in heart cells in type 1 diabetic subjects.