Evaluating Mirna-222 Expression Level and Its Association with AMH for Early Diagnosis of Pcos-Like Symptoms in Epileptic Patients Plasma Treated with Sodium Valproate: A Case – Control Study

Mahya Rajabi; Seyed Mohsen Miresmaili; Fatemeh Montazri; Mahsa Nasresfahani; Seyed Jalal Zieai; Seyed Mehdi Kalantar

doi:10.18502/ssu.v29i10.8211

Mahya Rajabi Department of Biology, Science and Arts University, Yazd, Iran.
Seyed Mohsen Miresmaili Department of Biology, Science and Arts University, Yazd, Iran.
Fatemeh Montazri Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mahsa Nasresfahani Department of Biochemistry, Payame Noor Taft University, Yazd, Iran.
Seyed Jalal Zieai Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Seyed Mehdi Kalantar Infertility Research Center, Yazd Reproductive Sciences Research Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.

DOI: https://doi.org/10.18502/ssu.v29i10.8211

Keywords: Epilepsy, Sodium valproate, miRNA-222, PCOs, AMH

Abstract

Introduction: Epileptic neurological disorder, which is controlled with medications such as sodium valproate (one of the treatment priorities for the patients with epilepsy). Polycystic ovary syndrome (PCOS) is one of complication of sodium valproate. Ovulation in PCOs Patients is disrupted, resulting in serious complications, including endometrial hyperplasia with typical and atypical forms, increased the risk of endometrial endometrial cancer, diabetes mellitus, and decreased fertility rates. This study, in accordance with bioinformatics studies and other studies based on the association of miRNA-222 with genes involved in PCOs and hyperandrogenism, was designed to evaluate the association of PCOs in the patients treated with sodium valproate by simultaneously comparing the AMH factor and miRNA-222 marker at specific time periods.

Methods: In this case-control study, 33 women with epilepsy before and after use the drug were selected based on inclusion and exclusion criteria. After blood sampling, their plasma was isolated. According to the instructions of the Total RNA extracted kit , cDNA synthesized and miRNA-222 expression was evaluated by RT-qPCR technique and statistical analysis was performed by SPSS 23 software, T-test and ANOVA tests.

Results: The results of statistical tests such as T-test and ANOVA test (done with SPSS software) which showed a significant difference (p<0/01) between the mean expression of mir-222 and AMH in the patients before treatment compared to 3 month after treatment. The results of Pearson correlation test showed that the increase in AMH is directly related to the decrease in miRNA-222 expression (p<0/01).

Conclusion: According to the results of the present study, in order to better control the possible side effects of the drug and possibly timely drug change and early diagnosis of PCOs (PCOs‌-like symptoms), evaluation of miRNA-222 expression changes can be used at the same time with AMH assay.