Evaluation of NOS2 Polymorphism in Gastric Adenocarcinoma Patients in Mazandaran Province

Mahshad Hooshyar; Saeed  Abedian-Kenari; Abbas Mohammadpour; Habibeh Mirmajidi; Majid Jafari-Sabet; Ramin Ataee

doi:10.18502/ssu.v29i6.6994

Mahshad Hooshyar Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Saeed Abedian-Kenari Immunogenic Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Abbas Mohammadpour Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.
Habibeh Mirmajidi Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
Majid Jafari-Sabet Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran
Ramin Ataee Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.

DOI: https://doi.org/10.18502/ssu.v29i6.6994

Keywords: NOS2, Gastric cancer, PCR-RFLP, Polymorphism.

Abstract

Introduction: Gastric cancer is the fourth most common cancer and the second leading cause of cancer death worldwide. North of Iran is a high-risk area for gastric cancer. Nitric oxide (NO), mainly synthesized by inducible nitric oxide synthase (NOS2) in pathological conditions, plays an important role in cytotoxicity, inflammation and fibrosis. In this research we studied the effect of (rs1137933) T>C genotype on gastric cancer.

Methods: This analysis was performed on 93 patients with gastric cancer who were referred to endoscopy Tuba Clinic in 2015 and 93 healthy individuals as controls. DNA extracted from peripheral blood samples was applied in PCR-RFLP (Polymerase chain reaction- restriction fragment length polymorphism) analysis to determine (rs1137933) T>C genotype. The association of the (rs1137933) T>C genotype and gastric cancer risk were analyzed by MedCalc software and (X2) Chi-square and (OR) Odds Ratio exams.

Results: Frequency of TT, CT, and CC genotypes in cases was 12.61, 51.35 and 36% and 38.7, 34.4, and 26.8% in the control group. Significant association was found between (rs1137933) T>C genotype with gastric cancer chance, P<0.05, OR=2/04, 95% CI (1/37 to 3/03).

Conclusion: The results of the study show that the presence of CC+CT genotypes may increase the risk of gastric cancer. P < 0.0001, OR=4.37 (2.17 to 8.80).Therefore, investigating the (rs1137933) T>C single nucleotide polymorphism of NOS2 gene could be an appropriate molecular marker that could be used to determine individual sensitivity to gastric cancer and for designing cancer prevention programs.