Frequency of fimH, papC, and aer in E. coli Strains Isolated from Patients with Urinary Tract Infection, and their Antibiotic Resistance Pattern

Zahra Abdolahzadeh; Akram Astani; Ahmad Mosadegh; Saeede Sadat  Hosseini Mohammad Abadi; Mahmoud Vakili

doi:10.18502/ssu.v29i3.6200

Zahra Abdolahzadeh Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
Akram Astani Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
Ahmad Mosadegh Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
Saeede Sadat Hosseini Mohammad Abadi Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.
Mahmoud Vakili Department of Microbiology, School of Medicine, Shahid Sadoghi University of Medical Sciences and Health Services, Yazd, Iran.

DOI: https://doi.org/10.18502/ssu.v29i3.6200

Keywords: Escherichia coli, UTI, virulence factor, aer, papC, fimH, Antibiotic Resistance.

Abstract

Introduction: E. coli is the predominant causes of urinary tract infection. Several virulence factors for bacterial infections in the urinary tract are required. In this study, we have considered several virulence factors in strains isolated from the patients with UTI in Yazd.

Methods: In this cross-sectional study in 1394-1395, 146 strains isolated were collected from the patients with urinary tract infection. After confirmation by phenotypic and genotype methods, frequency of gene fimH, pap C, aer was evaluated using specific primers by PCR method. The pattern of antibiotic resistance of isolates was determined by disk diffusion method.

Results: In the present study, the prevalence of virulence genes was fimH (87%), aer (85.6%) and papC (9.6%). Among 146 E. coli isolates, resistance rate for various antibiotics were as follows: 57.2% to Cotrimoxazole, 54.8% to Nalidixic acid, 45.9% to Cefazolin, 40.4% to Cefixime, 42.5% to Cefalotin, 41.8% to Cefalexin, 31.5% to Norfloxacin, 30.1% to Ofloxacin, 28.3% to Ciprofloxacin, 24% to Gentamicin, 19.9% to Amikacin, 1.4% to Nitrofurantoin, and 1.4% to phosphomycin. The results showed that the most and less frequent resistant was seen in Cotrimoxazole and Nitrofurantoin, phosphomycin, respectively.

Conclusion: In this study, the frequency of fimH gene was higher than other genes. In addition, according to the pattern of antibiotic resistance nitrofurantoin and phosphomycin are suitable medicines for the treatment of patients in our region.