Effect of Voluntary Training after the Induction of Experimental Autoimmune Encephalomyelitis on Some Myelin-Producing Proteins in Female C57BL/6 Mice

Abstract

Introduction: The aim of the present study was to investigate the effect of voluntary training period after the induction of experimental autoimmune encephalomyelitis (EAE) on some myelin-producing proteins in C57BL/6 female mice.

Methods: In this experimental study first 28 mice, which were 6-8 weeks old, were purchased and were randomly divided into three groups. Exercise activity (n=12), healthy control (n=8) and experimental autoimmune encephalomyelitis (EAE) control (n=8). Voluntary exercise group did exercises, 1 hour, 5 days a week for 4 weeks after induction of EAE and having the clinical score one for two days in a row. 48 hours after final exercise section, the mice were killed and immunohistochemistry was used to evaluate the expression of MBP and CNPase proteins. Using SPSS version 16 software, multiple analysis of variance and LSD post hoc test were used to examine the groups' data differences.

Results: The results showed that the expression of both proteins as a result of voluntary exercise had a significant increase in the exercise group compared to the EAE control group ( p<0.05).( in White Matter: MBP, Wheel Running and Control EAE, P= .017; Wheel Running and Healthy Control, P= .001; CNPase, Wheel Running and Control EAE, P= .015; Wheel Running and Healthy Control, P= .000; in Gray Matter: MBP, Wheel Running and Control EAE, P= .000; Wheel Running and Healthy Control, P= .000; CNPase, Wheel Running and Control EAE, P= .005; Wheel Running and Healthy Control, P= .001.

Conclusion: Voluntary exercise may have a positive effect on increasing myelination in treatment and control of MS.