Investigation of IL-17Agene Polymorphism (Rs2275913) with Infertility in Southern Iranian Women

Abstract

Introduction: Estimates show that about 3-7% of all couples or women around the world have an unsolved infertility problem. Interleukin-17 (IL-17) is a pro inflammatory cytokine involved in the expression of many inflammatory mediators. The aim of this study was to investigate the relationship between (rs2275913G/A) IL-17A gene polymorphism with infertility in women.

Methods: This case-control study was carried out on 200 patients with infertility and 200 healthy individuals whose conditions were confirmed by an infertility specialist. Genomic DNA was extracted from peripheral blood using the Salting Out method and K Proteinase. To examine the position of rs2275913 polymorphism the RFLP-PCR method was used. Data were statistically analyzed using SPSS software Ver. 20, Chi-square and Hardy-Weinberg tests. The P-values ≤ 0.05 was considered significant.

Results: The results showed that allele A at position rs2275913 had a significant correlation with infertility (P = 0.000). It was found the patients with polycystic ovarian syndrome (P = 0.02) and Falobian tube disorders (P = 0.03) showed a significant relationship with rs2275913 Polymorphism.

Conclusion: According to the results, it appears that the rs2275913 polymorphism of IL-17 gene is related to the infertility in women.