Investigating the Effects of Epicatechin on Cypermethrin-Induced Oxidative Stress and Genotoxicity on PC-12 Cell Line

Negar  Azarinegad; Elahe  Gharehkhani; Mahboube  Rahmati; Mohammad  Shokrzadeh

doi:10.18502/ssu.v34i1.21524

Negar Azarinegad Ramsar Campus, Mazandaran University of Medical Sciences, Ramsar, Iran
Elahe Gharehkhani Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Mahboube Rahmati Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Mohammad Shokrzadeh Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran

DOI: https://doi.org/10.18502/ssu.v34i1.21524

Keywords: Epicchin, Oxidative stress, Genotoxicity, Cypermetrine

Abstract

Introduction: Epicatechin is recognized as a novel and potent antioxidant with significant potential for the treatment of neurological diseases. However, prior to its clinical application, any a pharmacological compound must first be evaluated through preclinical research. Considering the above, we decided to investigate the effects of epicatechin on oxidative stress and genotoxicity induced by cypermethrin in the PC-12 cell line.

Methods: In this study, PC-12 cell line was pretreated with different concentrations of epicatechin (50, 100, 200, 400, and 800 μg/ml), followed by exposure to cypermethrin (129.3 μM) to induced cytotoxicity. Subsequently, several parameters, including micronuclei number, oxygen free radicals, cell viability, and glutathione level were measured.

Results: In the present study, epicatechin was able to significantly reduce the number of micronuclei and increase cell viability at concentrations of 50, 100, 200, 400, and 800 μg/mL. However, at the lowest concentration (50 μg/mL), it did not significantly increase glutathione levels or reduce the level of oxygen free radicals. In contrast, concentrations of 100, 200, 400, and 800 μg/mL significantly improved the aforementioned parameters.

Conclusion: In general, it seems that epicatechin at different concentrations can improve oxidative stress and xenotoxicity parameters in the PC-12 cell line