Evaluation the influence of Spirulina platensis extract on cell viability and change the expression of apoptotic BAD gene in the AGS human gastric adenocarcinoma cells

Abstract

Introduction: In this study, the influence of Spirulina platensis extract with anti-inflammatory, cancer prevention and immune system strengthening properties on cell viability and changes in BAD apoptotic gene expression in human gastric adenocarcinoma cells of  the AGS line was investigated.

Conclusion: The results indicate that Spirulina platensis algae extract is able to reduce the cell survival of gastric adenocarcinoma cells by inducing BAD gene expression and preventing proliferation.

Methods: In the present experimental study, by culturing the AGS cell line in DMEM-12 medium with 10% fetal bovine serum and treating the cells at concentrations of 10, 20 and 40 μg/ml of Spirulina platensis algae extract and incubating for 24 and 48 hours, cytotoxicity was examined using the MTT colorimetric method according to the kit instructions at both incubation times. The AGS line cells were then incubated  with a concentration of Spirulina platensis extract at IC50 point (40 µg /mL) for 48 hours to assess the expression level of the BAD gene via real-time RT-PCR. Statistical analysis was accomplished using Mann-Whitney test using SPSS 16 software.

Results: The results of this study indicated that the cell viability of AGS gastric Adenocarcinoma cell line significantly decreased at concentration of 20 (P<0.001) and 40 (P<0.0001) µg/mL compared to the control group. In addition, AGS cell line treatment with Spirulina platensis extract at a dose of 40 µg/mL and 48 incubation time, showed a significant increased expression of BAD gene (P<0.001).

Conclusion: The results indicate that Spirulina platensis extract can reduce the viability of gastric adenocarcinoma cells by inducing expression of BAD gene that preventing proliferation