Effect of Eight Weeks of Combined (Resistance - Endurance) Training and Taurine Supplementation on Protein Expression of LXR Receptors and Total Cholesterol Serum Level in Diabetic Male Wistar Rats

Ameneh PourRahim  Ghouroghchi; Aydin Valizadeh  Orange

doi:10.18502/ssu.v32i12.17943

Ameneh PourRahim Ghouroghchi Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
Aydin Valizadeh Orange Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

DOI: https://doi.org/10.18502/ssu.v32i12.17943

Keywords: Resistance-endurance training, Taurine supplementation, LXR protein expression, Serum total cholesterol, Diabetic rats.

Abstract

Introduction: Liver X Receptor is a crucial gene in liver responsible for maintaining glucose and cholesterol homeostasis. Diabetes leads to disruption in LXR and total cholesterol disorder. The aim was to determine the effect of eight weeks of combined training (resistance-endurance) along with taurine supplementation on the expression of LXR receptors and serum total cholesterol in male Wistar rats.

Methods: The participants in this experimental study consisted of 50 male Wistar rats, each 6 weeks old, weighing between of 200-220 grams. To induce diabetes of 40 rats, at the end of the eighth week, the amount of 55 mg of streptozocin (STZ) per rat’s body weight was injected intraperitoneally, and the rats were randomly divided into four groups: combined exercise (n=10), taurine (n = 10), combined training and taurine (n = 10) and control (n = 10). Ten healthy rats were considered as healthy controls. The combined training group completed resistance training, which included climbing the ladder 15 times, along with endurance workouts on the treadmill at an intensity of 75% Vo2max for eight weeks, 5 times each week. The taurine group received 1% taurine supplement solution in drinking water daily. Exercise and taurine interventions were applied in the combined exercise-taurine group. Two-way ANOVA along with the LSD post hoc test was employed to compare intergroup and intragroup.

Results: After eight weeks, LXR levels decreased significantly in the supplement group (P=0.0001) and the exercise + supplementation group (P=0.0001) when compared to the control group. LXR showed a notable reduction in the exercise + supplement (P=0.0001) and supplement (P=0.011) groups when compared to the exercise group. Cholesterol levels decreased significantly in the exercise + supplement group (P=0.027) when compared to the control group. Body weight and BMI significantly were lower in the exercise, supplement and exercise + supplement groups in comparison to the control group. Although it was significantly higher in the exercise group when compared to the supplement and exercise + supplement groups (P<0.05).

Conclusion: Eight weeks of combined (resistance-endurance) training and taurine consumption decreased LXR, body weight, BMI and total cholesterol in diabetic rats.