Studying the Pathogenic Effect of New Nucleotide Changes in the Promoter Region of the TERT Gene in Patients with Glioblastoma Brain Tumor

Farzaneh Owlia; Mohammad Mehdi Heidari; Mehri Khatami; Ehsan Ziaei; Mohammad Ali Broomand

doi:10.18502/ssu.v32i3.15545

Farzaneh Owlia Department of Biology, Yazd University, Yazd, Iran.
Mohammad Mehdi Heidari Department of Biology, Yazd University, Yazd, Iran.
Mehri Khatami Department of Biology, Yazd University, Yazd, Iran.
Ehsan Ziaei Department of Neurosurgery, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mohammad Ali Broomand Clinical Oncology Department, Shahid Ramazan Zadeh Clinic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

DOI: https://doi.org/10.18502/ssu.v32i3.15545

Keywords: Brain tumor, Telomerase, Glioblastoma multiform, TERT gene, Touchdown PCR, Bioinformatics.

Abstract

Introduction: Brain tumors are considered to be one of the most dangerous types of cancer, despite decades of research and treatment efforts. The activation of the telomerase enzyme is a critical factor in the immortality of these cells. Mutations in the promoter region of the TERT gene, particularly in the promoter hot spots, can influence the binding of activating transcription factors and inhibit the binding of negative regulatory factors of the TERT gene, ultimately regulating the gene's expression. This study aimed to identify and investigate the mutations of the TERT gene promoter region in glioblastoma, a malignant brain tumor, using bioinformatics.

Methods: A study was conducted using the case-control method where 35 patients with glioblastoma multiform brain tumor were examined along with 40 people who were examined as control samples. In this research, the Touchdown PCR technique and direct DNA sequencing were used to detect mutations in the promoter region of the TERT gene in patients with glioblastoma. Furthermore, bioinformatic analyses were conducted to investigate the pathogenic effect of nucleotide changes in this gene region.

Results: In the core region of the TERT gene promoter, three-point mutations were identified (c.*146C>T، c.*144C>G and c.*143G>C). Two of these mutations were novel and have been observed for the first time in glioblastoma multiform. The remaining mutation was located in the promoter core's hot spot of the gene. This mutation was known as c.*146C>T.

Conclusion: In this research, the harmful impact of TERT promoter region mutations as a biomarker for glioblastoma was examined. These findings suggest that mutations in regulatory regions, as well as coding sequences, could be significant contributory factors in the process of carcinogenesis.