Evaluation of the Relationship between Vitamin D Receptor Gene Polymorphisms and Multiple Sclerosis Patients Who Responsive or non-Responsive to Treatment with Beta Interferon

Zeinab Fatahi; Morteza Samadi; Reyhaneh Sadat Sandoghsaz; Reza  Najafipour; Safar Ali Alizadeh

doi:10.18502/ssu.v31i3.12807

Zeinab Fatahi Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Morteza Samadi Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isafahan, Iran.
Reyhaneh Sadat Sandoghsaz Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Reza Najafipour Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Safar Ali Alizadeh Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

DOI: https://doi.org/10.18502/ssu.v31i3.12807

Keywords: Multiple sclerosis, Vitamin D receptor gene, Beta-interferon.

Abstract

Introduction: Multiple Sclerosis is a chronic, inflammatory disorder of the central nervous system, which leads to demyelination of nerves. Treatment with interferon beta is one of the best approaches to reduce recurrence and progression of the disease. The aim of this study is to search for the relationship between 4 polymorphisms FokIrs10735810, TaqIrs731236, ApaIrs797532 and BsmIrs1544410 located in the vitamin D receptor in MS patients responsive and non-responsive to interferon beta treatment and pave the way for the correct and fast administration of the appropriate drug, as well as reducing time, cost and lack of success in treatment.

Methods: The type of study is observational and the study method is case-control. Blood samples were taken from 200 MS patients who were treated with interferon (100 responders and 100 non-responders). Using PCR-RFLP technique, the samples were subjected to enzymatic digestion with restriction enzymes ApaI, FokI, TaqI and BsmI. The data were evaluated using SPSS version 16, SNP Analyzer and Prism software, and K-tests and logistic regression.

Results: A significant difference in the genotypic and allelic frequencies of ApaI (p=0.68), BsmI (p=0.65), FokI (p=0.57) and TaqI (p=0.50) polymorphisms in the patients between the two responsive and non-responsive groups to treatment was not observed.

Conclusion According to our findings, there was no significant correlation between ApaI, FokI, TaqI and BsmI polymorphisms located in vitamin D receptor gene and response to treatment.