Effects of Lipoherb with Metformin on Metabolic Parameters and Sex Hormones in Rats with Polycystic Ovary Syndrome

Fatemeh Tavakoli; Azadeh Emami; Behrooz Heydari; Mohsen Abbasi; Mohammad Ebrahim Rezvani

doi:10.18502/ssu.v30i12.11962

Fatemeh Tavakoli Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Azadeh Emami Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Behrooz Heydari Department of Clinical Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mohsen Abbasi Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mohammad Ebrahim Rezvani Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

DOI: https://doi.org/10.18502/ssu.v30i12.11962

Keywords: Polycystic ovary syndrome, Letrozole, Lipoherb, Metformin.

Abstract

Introduction: The purpose of the present study was comparing the effects of Lipoherb with metformin on metabolic changes and sex hormones of rats with polycystic ovary syndrome

Methods: Thirty female Wistar rats were divided into 5 groups (n=6). Group (1): received normal saline for 56 days, group (2): received letrozole (1mg/kg) for 28 days and normal saline for the next 28 days, group (3): in the first 28 days, they received letrozole carrier and in the next 28 days, they received Lipoherb (320 mg/kg), group (4): they received letrozole (1mg/kg) for 28 days and Lipoherb (320 mg/kg) in the next 28 days, group (5): they received letrozole (mg/kg) for 28 days and metformin (250 mg/kg) in the next 28 days. Letrozole at the dose of 1 mg/kg for 28 days induced polycystic ovary syndrome. At the beginning and at the end of the experiment, fasting blood sugar level, lipid profile and sex hormones were measured by ELISA method.

Results: Blood sugar in the letrozole plus metformin group was significantly lower than letrozole group (P<0.01). The amount of serum triglyceride and LDL cholesterol in the group that received letrozole and Lipoherb were significantly decreased compared to letrozole group (P>0.05). In the same group, total cholesterol concentration also decreased significantly compared to other groups (P>0.01). The serum level of estrogen in the group received Lipoherb was significantly lower compared to letrozole group (P<0.05).

Conclusion: Compared to metformin, Lipoherb had more ability to correct the metabolic symptoms of rats with polycystic ovary syndrome. However, the hormonal effects of Lipoherb did not differ significantly from the hormonal effects of metformin in polycystic ovary syndrome.