Effect of six weeks of resistance training on the muscle proteasome activity and autophagy flux in mice with cancer induce cachexia

Abstract

Introdution: Muscle weakness that is caused by cancer called Cachexia. One of the causes of the formation of the cachexia is the change in protein degradation, and the ubiquitous protease and Autophagy Lysosomes system is the most important protein breakdown system. Resistance training has been one the best stimulator of increasing muscular mass. Therefore, the purpose of this study was to investigate the effect of a period of resistance training on the muscle protease activity and autophagy flux in mice with cancer induce cachexia.

Methods: This study was conducted on 12 male BALB / c mice (age 6 weeks) and CT-26 tumor implanted into them. Then they were divided into two groups of resistance training (n=6) and control (n=6). Training group performed 6 weeks progressive resistance training. The protease activity was evaluated by fluorogenic substrate method and LC3B and p62 evaluated by western blot method. Independent t-test was carried out at P<0.05 for statistical analysis using Prism (7) software.

Results: There was no significant difference in Proteasome activity (p=0.13), muscle weight (p=0.24), and CT26 tumor volume (p≥0.05) between two groups of resistance training and control, but p62 decreased in the resistance training group (p=0.032).

Conclusion: However, the autophagy flux has improved in the part of cargo breakdown with resistance training, but perhaps the presence of a tumor inhibits muscle response to resistance training or this type of resistance training is not an appropriate intervention for treating cachectic muscle. To treat cachexia through the resistance training, either this method would be combined with other interventions or other methods of the resistance training can be applied.