Effect of 4 Weeks Preconditioning with High Intensity Interval Training and Moderate Intensity Continuous Training on Brain Levels of TNF-Α and TLR4 in Male Wistar Rats

Abbas  Foroughi Pordanjani; Mohsen  Salesi; Rasoul  Rezaei; Javad  Nemati

doi:10.18502/ssu.v30i10.11451

Abbas Foroughi Pordanjani Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran.
Mohsen Salesi Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran.
Rasoul Rezaei Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran.
Javad Nemati Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran.

DOI: https://doi.org/10.18502/ssu.v30i10.11451

Keywords: HIIT, MICT, Preconditioning, TNF-α, TLR4.

Abstract

Introduction: Inflammation in the brain is known as neuroinflammation and increases TNF-α (Tumor necrosis factor alpha) secretion and activates the TLR4 (Toll-like receptor 4) pathway. The aim of this study was to evaluate the effect of 4 weeks preconditioning with high intensity intermittent training (HIIT) and moderate intensity training (MICT) on TNF-α and TLR4 levels in male Wistar rats.

Methods: The current research was an experimental study.18 male Wistar rats were divided into three groups: high intensity intermittent training (6 heads), continuous moderate intensity training (6 heads) and the control group (6 heads). HIIT program included 6 stages of 2-minute activity with intensity of 85-90% VO2max and 5 stages of active rest for 2 minutes and MICT program with intensity of 65% VO2max with the same duration as HIIT for 4 weeks and 5 days per week was implemented. 48 hours after the last training session, rats were dissected and cortical tissues and layers of mice were extracted for TNF-α and TLR-4 using western blotting. Data analysis was performed using ANOVA test at a significance level of 0.05 With the help of SPSS version 16 software.

Results: The results of the present study showed that TNF-α and TLR4 in the cortex and stratum cerebral areas after 4 weeks of high-intensity and moderate-intensity intermittent aerobic exercise did not change significantly compared to the control group (p≥0.05).

Conclusion: According to these findings, it can be stated that exercise with high or moderate intensity cannot be an effective factor in increasing inflammatory markers in the brain.