Effect of 4 Weeks of High Intensity Interval Training with Curcumin Supplementation on COL-I, COL- II, SMAD3 Gene Expression in the Myocardium of Obese Male Rats with Type 2 Diabetes

Sara Fereshtian; Maghsoud Peeri; Hamid Agha Alinejad; Maryam Delfan

doi:10.18502/ssu.v30i8.11012

Sara Fereshtian Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Maghsoud Peeri Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Hamid Agha Alinejad Department of Exercise Physiology, Tarbiat Modares University, Tehran, Iran.
Maryam Delfan Department of Exercise Physiology, Alzahra University Sport Sciences, Tehran, Iran.

DOI: https://doi.org/10.18502/ssu.v30i8.11012

Keywords: High intensity interval training, Curcumin supplement, Type2 Diabetes, SMAD/3, COL-I, COL-II.

Abstract

Introduction: The aim of this study was investigating the effect of 4 weeks of high intensity interval training with curcumin supplement on the gene expression of collagen I, II and SMAD/3 in the myocardium of male obese rats with type 2 diabetes.

Methods: In this experimental study, 35 male diabetic rats were divided in to 5 groups of seven; normal control, diabetic control, curcumin+diabetic, high intensity interval training, high intensity interval training+curcumin. Diabetes was induced by an intraperitoneal injection of streptozotocin with a dose of 60 mg/kg in all groups except the normal control group. Curcumin was gavage 200 mg/kg of body weight in all groups except normal control and diabetic control groups to determine the expression of COL-I, II and SMAD/3 genes, PCR-Real time method and group comparison were used by Two-way ANOVA test at alpha level of 0.05.

Results: The decrease in COL-I gene expression in (S+HIIT (group compared to the (DC) (P<0.0001) and S+DC (P<0.0004) was significant, respectively. In high intensity interval training (HIIT) group, the decrease in COL-I gene expression was also significant compared to the diabetic control (DC) group (P=0.017).The decrease in COL-II gene expression in curcumin + high intensity interval training (S+HIIT) group compared to the (S+DC) (P=0.001) and diabetic control (DC) (P<0.0003) and in (HIIT) group was significant compared to the (DC) group (P=0.013) and (S+DC) (P=0.029).The decrease in SMAD/3 gene expression in (S+HIIT) group compared to the HIIT (P=0.008), (S+DC) (P<0.0002), (DC) groups (P<0.0006) and in the (HIIT) group was significant compared to the (NC) (P=0.010), (DC) (0.006) and (S+DC) groups (P=0.032).

Conclusion: The results showed that, high intensity interval training reduced COL-I, COL-II and SMAD/3 genes and decreasingthe expression of SMAD/3 due to high intensity interval training with curcumin supplementation possibly can improve diabetic fibrosis in myocardial.