Evidence of Crimean-Congo Haemorrhagic Fever Virus Occurrence in  Ixodi¬dae Ticks of Armenia

Hasmik   Gevorgyan; Gohar G.  Grigoryan; Hripsime  A. Atoyan; Martin   Rukhkyan; Astghik   Hakobyan; Hovakim   Zakaryan; Sargis  A. Aghayan

doi:10.18502/jad.v13i1.928

Hasmik Gevorgyan
Gohar G. Grigoryan
Hripsime A. Atoyan
Martin Rukhkyan
Astghik Hakobyan
Hovakim Zakaryan
Sargis A. Aghayan

DOI: https://doi.org/10.18502/jad.v13i1.928

Keywords: CCHF; Ixodid ticks; Armenia; ELISA; Infection rate

Abstract

Background: Crimean-Congo hemorrhagic fever (CCHF) causes serious health problems in humans. Though ticks of the genera Hyalomma play a significant role in the CCHF virus transmission it was also found in 31 other tick species.

Methods: Totally, 1412 ticks from 8 remote sites in Armenia during 2016 were sampled, pooled (3-5 ticks per pool) and tested for the presence of CCHFV antigen using ELISA test.

Results: From 359 tick pools, 132 were CCHF virus antigen-positive. From 6 tick species, four species (Rhipicephalus sanguineus, R. annulatus, R. bursa, Hyalomma marginatum) were positive for the virus antigen and R. sanguineus was the most prevalent (37.9%). Dermacentor marginatus and Ixodes ricinus revealed no positive pools, but both revealed delectable but very low virus antigen titers. The highest infection rate (50%) was observed in R. sanguineus, whereas H. marginatus rate of infection was 1 out of 17 pools.

Conclusion: For the first time in the last four decades CCHF virus antigen was detected in Ixodid ticks of Armenia. This finding substantiates the role of R. sanguineus in the disease epidemiology; however, the role of H. marginatum in the CCHF virus circulation in the country could not be excluded.