Effects of N-Acetylcysteine on FAS Gene Expression Level in Testicular Tissue of Acrylamide-Treated Adult Rats

E Shahrzad; M Shariati; S Naeimi; MA Edalatmanesh

doi:10.18502/jabs.v11i2.8777

E Shahrzad Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran
M Shariati Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
S Naeimi Department of Genetics, Kazerun Branch, Islamic Azad University, Kazerun, Iran
MA Edalatmanesh Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

DOI: https://doi.org/10.18502/jabs.v11i2.8777

Keywords: Apoptosis, N-acetylcysteine, Acrylamide, Testis, Rat

Abstract

Background & Objectives: Acrylamide (ACR) is a chemical with toxic effects on various body tissues. The present study was conducted to investigate the antioxidant effect of N-acetylcysteine (NAC) on the level of testicular apoptosis in acrylamide-treated adult rats.

Material & Methods: Thirty-six adult male Wistar rats were randomly divided into 6 equal groups. The intact control group was without treatment, the positive control group (PC) received 50 mg/kg ACR by oral gavage, the negative control group (NC) received 40 mg/kg NAC intraperitoneally, the animals in experimental groups of 1 (EXP1), 2 (EXP2) and 3 (EXP3) received 10, 20 and 40 mg/kg NAC intraperitoneally, respectively, and then all groups received 50 mg/kg acrylamide by oral gavage. The treatment period in all groups was 28 days. At the end of the study, FAS mRNA expression level was measured by real-time PCR and testicular tissue was evaluated histopathologically.

Results: The PC group showed a significant increase in FAS gene expression level (p<0.05) and spermatogenic degradation compared to the intact control and NC groups. The EXP1 and EXP2 groups showed decrease in FAS gene expression level (p˃0.05) and spermatogenesis improvement in a dose-dependent manner while the EXP3 group exhibited a significant decrease in FAS gene expression level (p˂0.05) and complete spermatogenesis recovery compared to the PC group.

Conclusion: The findings indicate that ACR increases apoptosis and destroys spermatogenesis by increasing FAS gene expression levels. In contrast, at the maximum dose (40 mg/kg), NAC could inhibit ACR-induced apoptosis by reducing FAS gene expression and improves spermatogenesis in rats.