Regular Exercise Training Enhances Spatial Memory and Regulates Glucocorticoid System in Experimental Autoimmune Encephalomyelitis

M Hafedh; AH  Parnow; C Jalili; D I. Patel; I Tsunoda

doi:10.18502/jabs.v11i4.8632

M Hafedh Department of Exercise Physiology, Faculty of Sports Sciences, Razi University, Kermanshah, Iran
AH Parnow Department of Exercise Physiology, Faculty of Sports Sciences, Razi University, Kermanshah, Iran
C Jalili Medical Biology Research Center, Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran
D I. Patel Faculty of Nursing, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
I Tsunoda Department of Microbiology, Faculty of Medicine, Kindai University, Osaka, Japan

DOI: https://doi.org/10.18502/jabs.v11i4.8632

Keywords: Regular Exercise, Experimental Autoimmune Encephalomyelitis, Dexamethasone, Memory, Corticosterone

Abstract

Background & objective: Exercise has been shown to improve cognitive function in patients with multiple sclerosis (MS). Experimentally, glucocorticoids (GCs) treatment has been observed to improve cognitive deterioration in an autoimmune model for MS, experimental autoimmune encephalomyelitis (EAE). We aimed to determine the combined effect of exercise and 4 mg/kg of dexamethasone (Dex) for 4 weeks on spatial memory in EAE.

Materials & Methods: Rats with EAE were subjected to the Morris water maze (MWM) for four days and a prop test for one day. The prop test was repeated on day 40 post-induction (dpi). Rats were randomly assigned to one of four groups (10 rats per group): control EAE without treatment; EAE + dexamethasone, (EAE + Dex); EAE + exercise (EAE + Ex); and EAE+Dex+Ex. Rats receiving dexamethasone were administered 4 mg/kg injections daily for two weeks after EAE induction. Exercise training was initiated on a motorized treadmill 2 weeks before EAE induction and continued until 14 dpi. On day 41, animals were dissected and CORT level was assessed by enzyme-linked immunosorbent assay corticosterone kit.

Results: One-way analysis of variance (ANOVA) with repeated measures followed by a protected LSD post hoc test indicated that, EAE+Ex group increased body weight (P < 0.001) and it displayed a significantly lower CORT concentration (P <0.001) with delayed clinical score until day 13 dpi. Further EAE+Ex improved memory by time spent (p > 0.001) and swimming speed (p>0.002).

Conclusion: The protocol selected in this study was an effective treatment for the EAE model to improve spatial memory and regulate corticosterone concentrations.