Evaluation of Microleakage and Biocompatibility of Universal Adhesive Containing Titanium Dioxide Nanoparticles: A Comparative In Vitro Study

F Koohpeima; MJ Mokhtari; M Salari

doi:10.18502/jabs.v11i4.8629

F Koohpeima Department of Operative Dentistry, Biomaterial Research Center, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
MJ Mokhtari Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran
M Salari Department of Operative Dentistry, Biomaterial Research Center, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

DOI: https://doi.org/10.18502/jabs.v11i4.8629

Keywords: TiO2 NPs, Microleakage, Universal Bond, NIH-3T3

Abstract

Background & Objective: Although dental composites have undergone a high level of development in recent years, there are still difficulties including microleakage. The incorporation of nanoparticles (NPs) in dental materials can produce antibacterial effects, but the effect of TiO₂ NPs on microleakage has not yet been investigated. The present study aimed to determine the effect of TiO₂ NPs incorporation in the universal single bond and pretreatment with them on microleakage of clV cavities using both total-etch and self-etch approaches.

Materials & Methods: Standard clV cavities were prepared on the buccal surfaces of the sixty non-carious human molar teeth. The restoration of samples was carried out with 6 different methods and randomly divided into six groups of ten subjects. Microleakage in both gingival and occlusal margins was determined after placement in 2% basic fuschin solution for 24h. Biocompatibility of a universal single bond was evaluated by MTT assay.

Results: In both occlusal and gingival margins in all groups, the application of TiO₂ NPs was better than no application. We observed the relatively low cytotoxic effect of TiO₂ NPs incorporated in the universal adhesive on NIH-3T3 cell viability.

Conclusion: Regarding the limitations of the present investigation, using TiO₂ NPs with both total-etch and self-etch approaches to universal single bond may lead to a decrease in the microleakage of resin composite restorations.