Biocompatibility Analysis of Mouse Spleen-Derived Extracellular Matrix

Yasin  Ghabool; Mahshid  Reghbati; Nasser  Mahdavi-Shahri; Amin  Tavassoli

doi:10.18502/jabs.v14i3.16358

Yasin Ghabool Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Mahshid Reghbati Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Nasser Mahdavi-Shahri Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Amin Tavassoli Department of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

DOI: https://doi.org/10.18502/jabs.v14i3.16358

Keywords: Decellularization, Scaffold, Spleen tissue, Extracellular matrix, Mesenchymal stem cells

Abstract

Background & Objectives: This study aimed to construct a decellularized mouse spleen scaffold and evaluate its cellular compatibility in vitro using murine bone marrow-derived mesenchymal stem cells (BM-MSCs).

Materials & Methods: A combination of physical, chemical, and enzymatic treatments was employed for mouse spleen decellularization. These included multiple freeze- thaw cycles, the ionic detergent sodium dodecyl sulfate (SDS), and enzymatic trypsin. Histological and scanning electron microscopy analyses were conducted up to 7 days post-culture to assess the impact of decellularization and cellular adaptation to the spleen scaffolds.

Results: Histological studies revealed the attachment and penetration of BM-MSCs into the scaffolds on days 5-7 following cell seeding. Furthermore, cell migration into the scaffold was observed 5 days after the seeding process.

Conclusion: The decellularization approach utilized in this study proved to be effective and biocompatible, supporting the preservation and proliferation of BM-MSCs. These findings indicate its potential for spleen tissue engineering applications.