The Antibacterial Effect of Nickel Nanoparticles Against Streptococcus mutans Compared to Chlorhexidine

Ali Bazrafkan; Farzan Modaresi; Sahar Roostamifar; Shekoufeh Atashpour; Azita Azad; Abdolmajid  Ghasemian

doi:10.18502/jabs.v13i3.13216

Ali Bazrafkan Departments of Microbiology, Advanced Medical Sciences and Technology, and Central Laboratory Research, Jahrom University of Medical Sciences, Jahrom, Iran
Farzan Modaresi Departments of Microbiology, Advanced Medical Sciences and Technology, and Central Laboratory Research, Jahrom University of Medical Sciences, Jahrom, Iran
Sahar Roostamifar Oral and Dental Disease Research Center, Department of Oral & Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
Shekoufeh Atashpour Departments of Pharmacology, Advanced Medical Sciences and Technology, and Central Laboratory Research, Jahrom University of Medical Sciences, Jahrom, Iran
Azita Azad Oral and Dental Disease Research Center, Department of Oral & Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
Abdolmajid Ghasemian Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran

DOI: https://doi.org/10.18502/jabs.v13i3.13216

Keywords: Dental caries, Streptococcus mutans, Nickel nanoparticles, Chlorhexidine, antibacterial effect

Abstract

Background & Objectives: Due to the increasing trend of extensive antibiotic resistance among bacterial strains and side effects, seeking novel methods such as nanoparticles (NPs) is promising for infection eradication.

Materials & Methods: Eighteen Streptococcus mutans (S. mutans) clinical isolates were collected from dental plaques. Moreover, S. mutans ATCC25175 standard strain was obtained from Pasteur institute of Iran. Following preparation of nanoparticles, their antibacterial effects were assessed compared to chlorhexidine. The nickel NPs (Ni-NPs) was prepared and its antibacterial effect was compared to the 12% chlorhexidine. The minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively) of Ni-NP (dilution range: 0.125- 64µg/mL) were measured using broth microdilution method.

Results: The nickel NPs (Ni-NPs) was prepared and its antibacterial effect was compared to the 12% chlorhexidine. The minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively) of Ni-NP (dilution range: 0.125-64µg/mL) were measured using broth microdilution method. The MIC and MBC levels of Ni-NP against the clinical isolates ranged 2-16µg/mL and 4-16µg/mL, respectively. These values against the S. mutans ATCC27175 standard strain included 4 and 8µg/mL, respectively. Furthermore, the MIC and MBC of chlorhexidine against clinical isolates ranged 8-64 and 32-64µg/mL, respectively, while both included 64µg/mL against standard strain (p<0.001).

Conclusions: The results of this study outlined that Ni-NPs exert efficient antibacterial effect at nontoxic concentrations compared to 12% chlorhexidine.