Antimicrobial Activity and Wound Healing Properties of Aloe Arborescens Extract:  An in Vivo Study

Fatemeh Ghane; Pegah  Rasouli; Benafshe  Khanjari; Alireza Yousofi; Ali  Zarenezhad; Hossein Fattahi; Mahsa  Rostami Chijan; Abdolmajid Ghasemian; Behnoosh Miladpour; Elham  Zarenezhad

doi:10.18502/jabs.v13i2.12726

Fatemeh Ghane Department of Chemistry, Darab branch, Islamic Azad University, Darab, Iran
Pegah Rasouli Department of Chemistry, Fasa branch, Islamic Azad University, Fasa
Benafshe Khanjari Department of Chemistry, Darab branch, Islamic Azad University, Darab, Iran
Alireza Yousofi Department of Pathology, Faculty of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Ali Zarenezhad Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Hossein Fattahi Department of Microbiology, Faculty of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Mahsa Rostami Chijan Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Abdolmajid Ghasemian Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Behnoosh Miladpour Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fasa, Iran
Elham Zarenezhad Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran

DOI: https://doi.org/10.18502/jabs.v13i2.12726

Keywords: Wound healing; Burns, Herbal Medicines, Aloe Arborescens, in vivo

Abstract

Background & Objective: Burn is one of the prominent causes of death around the world, however drug discovery attempts for burn healing has not been entirely successful. Aloe arborescens (A. arborescens), is effective in the burning wounds healing and growth inhibition of bacterial pathogens. Our objective was to assess the wound healing and antibacterial effects of A. arborescens in vivo.

Materials & Methods: Thirty healthy Wistar rat animals were enrolled. The treatment process continued for 21 days and sampling was conducted on days 14 and 21 and the tissue slides were sent to the pathology laboratory for testing. The bactericidal activity of A. arborescens extract was evaluated using the disc diffusion method.

Results: A. arborescens demonstrated a significant effect on the healing of burn wounds. Furthermore, the antibacterial effects of the A. arborescens extract against Gram-negative (Escherichia coli, Pseudomonas aeruginosa) was significantly higher than that against Grampositive (Staphylococcus aureus, Bacillus cereus) bacterial species.

Conclusion: In conclusion, this study indicated that A. arborescens extract had an improving effect on the healing process of third degree burns without toxicity to the tissue.