Parvovirus B19 Infection May Potentially Determine the Fate of Hematopoiesis by Altering the Human Bone Marrow Mesenchymal Stem Cells Differentiation

Azin Elmi; Amir Atashi; Nematollah Gheibi; Shahin Amiri; Monireh Ajami; Mansoureh Ajami; Razieh Mohammadihaji; Naeimeh Khodabandeloo; Mehdi Azad

doi:10.18502/ijpho.v13i4.13770

Azin Elmi Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Amir Atashi Stem Cells and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
Nematollah Gheibi Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Shahin Amiri Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Monireh Ajami Department of Hematology, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Mansoureh Ajami Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran
Razieh Mohammadihaji Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Naeimeh Khodabandeloo Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
Mehdi Azad Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.

DOI: https://doi.org/10.18502/ijpho.v13i4.13770

Keywords: Adipocyte; Bone marrow; Mesenchymal stem cell; Osteoblast, Parvovirus B19

Abstract

Background: Human bone marrow mesenchymal stem cells (hBM-MSCs), as supporters for hematopoiesis, differentiate into osteoblasts and adipocytes. Studies showed that infection of hBM-MSCs by Parvovirus B19 (B19V) can affect the differentiation capability of hBM-MSCs. This study aims to evaluate B19V effects on the differentiation of hBM-MSCs.

Materials and Methods: In this experimental study hBM-MSCs were cultured up to passage 3. Nucleofection was subsequently employed to deliver a plasmid containing the B19V genome into the cells. The transfected cells were then differentiated into osteoblast and adipocyte lineages. qRT-PCR was then performed to analyze the differentiation 14 days after transfection.

Results: On the 14th day after induction the findings demonstrated a significant increase in adipocyte-specific (PPARγ and LPL) gene expression compared to the control group (p<0.05) and a slight but not statistically significant decrease in the expression of the osteocyte-specific genes (RUNX2 and osteocalcin) (p>0.05).

Conclusion: The results suggest that B19V infection can promote the differentiation of hBM-MSCs towards adipocytes and affect the bone marrow microenvironment as well as hematopoiesis