Raman Spectroscopic Characterization of Hepatic Differentiation of Mesenchymal Stem Cells

Sareh   Arjmand; Aram   Barzegar; Alemeh  Mohammadpour; Hanieh   Rezaei; Nahid  Davoodian; Hori  Ghaneialvar; Rasoul  Malekfar; Abbas  Sahebghadam Lotfi

doi:10.18502/ijml.v8i2.6276

Sareh Arjmand Protein Research Center, Shahid Beheshti University, Tehran, Iran
Aram Barzegar Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
Alemeh Mohammadpour Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Hanieh Rezaei Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
Nahid Davoodian Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Hori Ghaneialvar Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
Rasoul Malekfar Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
Abbas Sahebghadam Lotfi Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

DOI: https://doi.org/10.18502/ijml.v8i2.6276

Keywords: Adipose tissue, Hepatocyte-like cells, Mesenchymal stem cells, Raman spectroscopy

Abstract

Background and Aims: Mesenchymal stem cells (MSCs) are a preferred cell source for the generation of hepatocyte-like cells in regenerative medicine. They can be isolated from different sources, including adipose tissues. The Raman spectroscopy approach was evaluated for quick and efficient identification of MSCs differentiation status and a broader perspective on cell differentiation.

Materials and Methods: The human adipose-derived mesenchymal stem cells (hASCs) were differentiated toward hepatocyte-like cells using a well-established method. The cells were cultured on fluorescence-free quartz discs, and the efficiency of differentiation was examined using molecular and biochemical methods. The Raman spectra were recorded at days 1, 7, 14, and 21 of differentiation, and HepG2 was used as a positive control.

Results: The changes in Raman spectra were detected during the sequential stages of differentiation, and the pattern of peaks on the last day of differentiation was remarkably similar to the positive control (HepG2).

Conclusion: Raman spectroscopy showed considerable potential to characterize hepatic differentiation.