Evaluating the Distribution of (+ 2044G / A, R130Q) Rs20541 and (-1112 C / T) Rs1800925 Polymorphism in IL-13 Gene: An Association-Based Study with Asthma in Ahvaz, Iran

Abstract

Background and Aims: Asthma is a complicated chronic inflammatory disease associated with pulmonary inflammation, severe immune responses of the respiratory tract, and change or destruction in the respiratory tract structure. Several
factors, especially genetic and environmental factors, contribute to the pathogenesis of asthma. Interlukin (IL)-13 is considered the most important inflammatory mediator of asthma that play an effective role in the different stages of B cell
maturation and differentiation. It also increases the expression of major histocompatibility complex II and CD23 and is effective in IgE isotypes switching. Single nucleotide polymorphisms (SNPs) cause the wide genetic diversity in the
genome, and SNP analysis helps evaluate and diagnose disease-related genes. The present study was conducted to investigate the association of -1112C / T and + 2044 G /A polymorphisms in the IL-13 gene with asthma.

Material and Methods: Blood samples (5 ml) from asthma patients (167) and controls (172) after spirometry test were collected into tubes, and then DNA was extracted to determine the genotype of asthma patients. Real-time polymerase chain
reaction was performed by the Taq Man method, and the results were analyzed using SPSS software version 22.

Results: There was a significant difference between the genotype of IL-13 -1112C / T polymorphism in the patient group and the control group (p = 0.028). The IL13 + 2044G / A polymorphism results showed no significant difference between the two groups (p = 0.319).

Conclusions: The present study showed that IL-13 -1112C / T polymorphism positively correlates with the induction of asthma, but there was no significant association between the polymorphism + 2044G / A IL-13 and the risk of asthma.