The Oncogenic Potential of IQ Motif-Containing GTPase-Activating Protein3 in Human Bladder Cancer:  An In-Silico Analysis

Omid  Abazari; Serajoddin  Vahidi; Mohammad Hossein Modarressi; Javad Zavar  Reza

doi:10.18502/ijml.v11i1.16541

Omid Abazari Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Serajoddin Vahidi Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Hossein Modarressi Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Javad Zavar Reza Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

DOI: https://doi.org/10.18502/ijml.v11i1.16541

Keywords: Bioinformatics, Bladder cancer, IQGAP3, Oncogene

Abstract

Introduction: IQ motif-containing GTPase-activating protein3 (IQGAP3) contributes to the progression of bladder urothelial carcinoma (BLCA), but its mechanisms are not systematically specified. Due to the oncogenic potential of IQGAP3, the current in-silico study intended to elucidate IQGAP3's role in BLCA progression.

Materials and Methods: Many bioinformatics tools, including UALCAN, Kaplan–Meier plotter, TNMplot, cBioPortal, GeneMania, Enrichr, TIMER2, muTarget, and UCSC Xena, were applied in the current study.

Results: The IQGAP3 level was more pronouncedly raised in BLCA tissues than in normal bladder tissues, and its increased expression was related to the advanced stage and higher grade. Enhanced IQGAP3 expression could result from its genetic alteration. Moreover, the mutation in P53 and RB1 genes was robustly associated with increased IQGAP3 expression. Besides, IQGAP3 correlative genes were dominantly involved in the cell cycle. On the other hand, IQGAP3 upregulation influenced immune checkpoint levels in the tumor microenvironment.

Conclusion: The in-silico findings suggested that IQGAP3 overexpression could be a crucial biomarker in BLCA.