Comparison of the Effect of Temperature and Different Culture Media on the Possibility of Growth of  Salmonella Typhimurium (ATCC: 14028)

Nazila  Eslami; Younes  Anzabi; Mir Alireza  NourAzar

doi:10.18502/ijml.v10i4.15007

Nazila Eslami Department of Microbiology, Faculty of Basic Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Younes Anzabi Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
Mir Alireza NourAzar Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran

DOI: https://doi.org/10.18502/ijml.v10i4.15007

Keywords: Colony count microbial Gastroenteritis Pre-enrichment Salmonella infections

Abstract

Background and Aims: Gastrointestinal infection with Salmonella serotypes, especially Typhimurium, is common worldwide. One of the important points regarding the isolation of Salmonella is its similarity with other microbial flora, which makes it difficult to isolate it. Therefore, it is very important to choose the correct separation method. This study aimed to compare the growth rate of Salmonella in different cultural environments and the effect of temperature and enrichment on it.

Materials and Methods: 24 out of 36 Ross breed broilers were infected with the bacteria to obtain stool samples containing Salmonella Typhimurium. One McFarland dilution was given to chickens for experimental infection. After the confirmation of the infection, the culture was started in days for 7 consecutive days. From 2 media enriched with Tetrathionate broth and Selenite Cysteine broth and 3 selective culture media, Xylose lysine Deoxycholate agar, Brilliant Green agar, and McConkey agar for culture were used. All cultures were examined at 2 temperatures of 37°C and 42°C in terms of growth rate. The Kappa test was used for data analysis.

Results: The results showed no difference between tetrathionate and selenite cyane enrichment media (k=0.81). Agreement between Xylose Lysine Deoxycholate and McConkey (k=0.33), agreement between Xylose Lysine Deoxycholate and Brilliant Green (k=0.57), and agreement between Brilliant Green and McConkey (k=0.80), were obtained. The agreement between 37°C and 43°C was < 0.1.

Conclusion: According to the results obtained, using an Xylose Lysine Deoxycholate culture medium and incubation at 42°C are recommended to isolate Salmonella Typhimurium from fecal samples.